设计辅助反相高效液相色谱法测定商品食品样品中日落黄的分析质量：绿度、蓝度和白度评价

Green Analytical Chemistry Pub Date : 2024-12-05 DOI:10.1016/j.greeac.2024.100183

Atyurmila Chakraborty, Kavitha Jayaseelan

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引用次数: 0

摘要

日落黄FCF （E110）是一种广泛使用的偶氮合成食用色素，因为它具有吸引人的色调和显著的市场知名度。由于过量使用晚霞黄会带来健康风险，因此需要优先考虑对晚霞黄进行生态安全量化的方法。本研究的目的是建立并验证一种基于设计的高效液相色谱-超声辅助提取食品样品的方法，并用FTIR对其进行验证。乙醇具有良好的环境友好性，符合绿色分析化学的要求。采用旋转中心复合设计（rCCD）优化色谱参数，色谱柱为Phenomenex柱（C18, 250 mm × 4.6 mm，直径5 μm），流动相为34.4:65.6 （v/v）乙醇与乙酸缓冲液，流速1.1 ml/min，检测波长510 nm。日落黄的洗脱在2.133 min后进行。作为强制降解研究的一部分，对该方法进行了彻底的验证和测试，以确定其稳定性指示性质。在评估绿色程度时，绿色分析程序指数、分析生态尺度和AGREE指标工具产生了最好的结果。采用蓝色适用性等级指数（Blue Applicability Grade Index）和红绿蓝12 （Red-Green-Blue 12, RGB 12）算法分别对该方法的蓝度和白度进行评价，得分较高，表明该方法具有较高的实用性、成本效益和生态友好性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Analytical quality by design aided RP-HPLC method for the estimation of sunset yellow in commercial food samples employing green ultrasound assisted extraction: Greenness, blueness and whiteness evaluation

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Sunset Yellow FCF (E110) is a widely utillized azo synthetic food colorant due to its appealing hue and significant market popularity. Attributable to the health risks caused by excessive usage of Sunset Yellow, ecologically safe method for quantifying the same are to be prioritized. The purpose of this study is to develop and validate an Analytical Quality by Design based eco-friendly HPLC method followed by ultrasound assisted extraction of food samples and confirmation of them using FTIR. To comply with the requirements of green analytical chemistry ethanol was utilized because of its environmental friendliness nature. The chromatographic parameters were optimized using rotatable central composite designs (rCCD) whereas the separation was performed on a Phenomenex column (C₁₈, 250 mm × 4.6 mm, i.d 5 μm) using a mobile phase consisting of a 34.4:65.6 (v/v) mixture of ethanol and acetate buffer at flow rate of 1.1 ml/min with detection wavelength of 510 nm. The elution of Sunset Yellow occurred after 2.133 min. The method was thoroughly validated and tested as part of the forced degradation investigation to ascertain its stability-indicating properties. When evaluating the extent of greenness, the Green Analytical Procedure Index, Analytical Eco Scale and AGREE metrics tools yielded the best findings. The blueness and whiteness of the method was assessed by Blue Applicability Grade Index and Red-Green-Blue 12 (RGB 12) algorithm respectively with a high score which shows high practicality, cost effectiveness and ecofriendliness of the method.

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来源期刊

Green Analytical Chemistry

CiteScore

3.00

自引率

0.00%

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