内生芽孢杆菌n -2- 6s对乌头疫病和根腐病的有效防治

IF 3.7 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control Pub Date : 2025-02-01 DOI:10.1016/j.biocontrol.2024.105690

Qian Wang , Yujie Qiu , Siyu Wang, Yumei Gou, Hongji Hou, Tianwei Su, Lan Zou, Jing Huang

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引用次数: 0

摘要

摘要白叶枯病和根腐病是著名药用植物乌头（Aconitum carmichaelii Debeaux）的两种主要土传病害。以有益微生物为基础的生物防治已成为一种有前途和可持续的疾病防治替代方案。本研究从金莲茎中分离到一株内生细菌n -2- 6s。根据gyrA、rpoB和recA基因的串联序列进行系统发育分析，n -2- 6s为velezensis芽孢杆菌。双培养实验表明，n -2- 6s在离体和根片上均对罗氏菌核菌和尖孢镰刀菌表现出较强的拮抗能力。田间试验结果表明，n -2- 6s培养可显著降低白叶枯病和根腐病的发生，防效分别为91.00%和85.71%。生物防治效果至少持续54天。此外，与未接种和商业枯草芽孢杆菌处理相比，接种n- 2- 6s显著增加了草芽孢杆菌茎、主根和侧根的鲜重和干重。接种n -2- 6s显著提高了龙参侧根总生物碱积累量，达到17.36 mg/g。n -2- 6s体外培养滤液对罗氏弧菌和尖孢镰刀菌均有较强的抑制作用。PCR扩增结果显示，YN-2-6S具有ituC、fenA、fenB、fendf、srfAA、bmyB、bacA、baeA、mnlA和dfnA等基因，表明其具有抗真菌脂肽和多酮的合成能力。此外，n -2- 6s释放的挥发性化合物也显著抑制了S. rolfsii和F. oxysporum的体外生长。此外，n -2- 6s还能产生促进生长的葡聚糖酶、纤维素酶、蛋白酶、吲哚-3-乙酸。接种n -2- 6s后，柽柳叶片过氧化物酶（POD）和过氧化氢酶（CAT）活性升高，表明n -2- 6s可能诱导柽柳产生全身抗性。综上所述，本研究证明n -2- 6s是一种很有前景的防治南白叶枯病和根腐病的生物制剂，其作用机制可能涉及产生抗真菌的脂肽、聚酮、挥发性化合物、水解和诱导寄主的全身抗性。

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Effective control of southern blight and root rot of Aconitum carmichaelii Debeaux by endophytic Bacillus velezensis YN-2-6S

Southern blight and root rot were two main soil-borne diseases of Aconitum carmichaelii Debeaux, a famous medicinal plant rich in alkaloids. Beneficial microbe based biological control has been a promising and sustainable alternative for disease control. In this study, an endophytic bacterium YN-2-6S was isolated from the stem of A. carmichaelii. YN-2-6S was identified as Bacillus velezensis by phylogenetic analysis based on concatenated sequence of gyrA, rpoB, and recA genes. YN-2-6S showed strong antagonistic capacity against Sclerotium rolfsii and Fusarium oxysporum both in vitro and on root slices of A. carmichaelii by dual-culture assay. Field experiment revealed that YN-2-6S culture significantly reduced southern blight and root rot disease occurrences on A. carmichaelii, with 91.00 % and 85.71 % biocontrol efficiency respectively. The biocontrol activity persisted at least for 54 days. In addition, YN-2-6S inoculation significantly increased fresh and dry weight of stems, main roots and lateral roots of A. carmichaelii compared with non-inoculation and commercial B. subtilis treatments. YN-2-6S inoculation significantly promoted the accumulation of total alkaloids content of A. carmichaelii lateral roots to 17.36 mg/g. Cell-free culture filtrate of YN-2-6S showed strong inhibitory effect against S. rolfsii and F. oxysporum in vitro. The presence of ituC, fenA, fenB, fenD, srfAA, bmyB, bacA, baeA, mnlA, and dfnA genes by PCR amplification indicated that YN-2-6S was able to produce antifungal lipopeptides and polyketides. Furthermore, volatile compounds released by YN-2-6S also significantly inhibited the growth of S. rolfsii and F. oxysporum in vitro. In addition, YN-2-6S was able to produce glucanase, cellulase, protease, indole-3-acetic acid with growth-promoting ability. Increase of peroxidase (POD) and catalase (CAT) enzyme activity of A. carmichaelii leaves was detected upon inoculation of YN-2-6S, which indicated that YN-2-6S may induce systemic resistance on A. carmichaelii. Taken together, this study proved that YN-2-6S was a promising biocontrol agent against southern blight and root rot of A. carmichaelii and the underline mechanism may involve the production of antifungal lipopeptides, polyketides, volatile compounds, hydrolysis and induction of host systemic resistance.

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来源期刊

Biological Control 生物-昆虫学

CiteScore

7.40

自引率

7.10%

发文量

220

审稿时长

63 days

期刊介绍： Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.