以病人为中心的体积吸收显微取样后干血样的自动毛细管电泳分析

IF 6.5 Q1 CHEMISTRY, ANALYTICAL
Richard Maršala , Miloš Dvořák , Pavel Kubáň
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引用次数: 0

摘要

首次提出了一种自动分析容量吸收微采样(VAMS)采集的干血样本的方法。一个单一的商用毛细管电泳(CE)仪器(不需要硬件或软件修改)被用于快速分析干燥的材料使用几个完整的和新颖的特征。这些包括使用外部压力从Mitra®VAMS聚合物尖端制备亚分钟样品,使用取样尖端作为固有的微搅拌器进行即时洗脱均质,在CE出口制备/取样以更方便地调整毛细管长度,以及短端注射用于亚分钟CE分离和乳酸作为模型分析物的定量。对样品制备和分析的操作参数进行了全面评估,从而从所有内源性基质化合物中分离出乳酸盐。优化后的方法具有良好的峰面积内和日间重复性(RSD值≤7.2%),在临床相关浓度范围(0.15 ~ 20 mM)内线性(R²= 0.9990),检出限和定量限分别为0.03和0.1 mM,样品通量≥16个样品/小时。VAMS设备在环境条件下储存长达14天,未观察到老化。与便携式乳酸分析仪的比较分析显示,由于干燥过程中葡萄糖的糖酵解,干燥毛细血管血液中的乳酸浓度与液体毛细血管血液中的乳酸浓度略有升高,然而,Mitra®设备中的乳酸浓度与血浆中的乳酸浓度具有良好的相关性,这通常用于临床实践。本研究为vams采集的血液样本的自动分析提供了一个可靠、准确和环保的概念,并具有在以患者为中心的采样、临床诊断和研究以及个性化医疗保健方面的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Automated capillary electrophoresis analyses of dried blood samples after patient-centric volumetric absorptive microsampling

Automated capillary electrophoresis analyses of dried blood samples after patient-centric volumetric absorptive microsampling
An automated method for the analysis of dried blood samples collected by volumetric absorptive microsampling (VAMS) is presented for the first time. A single commercial capillary electrophoresis (CE) instrument (with no need for hardware or software modifications) was employed for the expeditious analyses of the dried material using several integral and novel features. These included the use of external pressure for sub-minute sample preparation from Mitra® VAMS polymeric tips, the use of the sampling tips as inherent micro-agitators for instant eluate homogenization, preparation/sampling at the CE outlet for more convenient capillary length adjustment, and short-end injection for sub-minute CE separation and quantification of lactate as a model analyte.
The operational parameters for the sample preparation and analysis were comprehensively evaluated resulting in a baseline separation of lactate from all endogenous matrix compounds. The optimized method demonstrated excellent intra- and inter-day repeatability of peak areas (RSD values ≤ 7.2 %), linearity (R² = 0.9990) over the clinically relevant concentration range (0.15 – 20 mM), the limits of detection and quantification at 0.03 and 0.1 mM, respectively, and a sample throughput ≥ 16 samples/hour. No ageing was observed for VAMS devices stored at ambient conditions for up to 14 days. Comparative analysis with a portable lactate analyser revealed slightly elevated concentrations in dried vs. liquid capillary blood due to the glycolysis of glucose during drying, nevertheless, lactate concentrations in Mitra® devices correlated well with those in blood plasma, which is typically used in clinical practice.
The present study offers a robust, accurate, and environmentally benign concept for the automated analyses of VAMS-collected blood samples with its potential application in patient-centric sampling, clinical diagnostics and research, and personalized healthcare.
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CiteScore
3.50
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