日本耐碳青霉烯临床粘质沙雷菌的遗传基础。

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2025-02-01 DOI:10.1016/j.jgar.2025.01.011

Hazim O. Khalifa , Shizuo Kayama , Mohammed Elbediwi , Liansheng Yu , Wataru Hayashi , Yo Sugawara , Mohamed-Yousif Ibrahim Mohamed , Hazem Ramadan , Ihab Habib , Tetsuya Matsumoto , Motoyuki Sugai

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引用次数: 0

摘要

目的：探讨1994 - 2016年日本临床粘质葡萄球菌碳青霉烯类耐药的遗传基础。从日本各地不同的医疗中心回收5135株粘质葡萄球菌临床分离株，在中心实验室进行鉴定，并使用肉汤微量稀释法进行抗菌药物检测。方法：所有对至少一种碳青霉烯类抗生素表现出中间或耐药表型的分离株均通过药敏试验和改良碳青霉烯类失活法（mCIM）进行碳青霉烯酶产酶鉴定。此外，通过全基因组测序对所有分离株进行了完整的遗传鉴定。结果：27株（0.53%）对厄他培南和/或美罗培南耐药。其中，10株菌株经mCIM试验表型证实为碳青霉烯酶产生菌。菌株对β-内酰胺类（48.1% ~ 100%）、两种氟喹诺酮类（77.8% ~ 88.9%）、替加环素和米诺环素（70.4%）、磺胺甲恶唑-甲氧苄啶（55.6%）等多种抗生素耐药。对所有碳青霉烯耐药菌株进行了全基因组测序，在8株分离株中发现blaIMP，其中7株含有blaIMP-1, 1株含有blaIMP-11，以及多个抗菌素耐药决定因素。重要的是，与国际粘质葡萄球菌分离株的系统发育比较揭示了遗传相关性和潜在的跨境传播事件。结论：我们的研究结果强调了加强监测和感染控制措施以减轻多重耐药病原体传播的重要性，强调了国际合作和协调努力解决全球范围内抗菌素耐药性问题的必要性。

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Genetic basis of carbapenem-resistant clinical Serratia marcescens in Japan

Objective

To investigate the genetic basis of carbapenem resistance in clinical Serratia marcescens isolates collected from patients in Japan between 1994 and 2016. A total of 5135 clinical isolates of S. marcescens were recovered from different medical centres across Japan, identified in central laboratories, and tested for antimicrobial agents using the broth microdilution method.

Methods

All the isolates that showed intermediate or resistant phenotypes for at least one carbapenem antibiotic were confirmed by antimicrobial susceptibility testing and for carbapenemase production by the modified carbapenem inactivation method. Furthermore, full genetic characterization was performed by whole genome sequencing for all the isolates.

Results

Based on our findings, 27 isolates (0.53%) exhibited resistance to ertapenem and/or meropenem. Among these, 10 isolates were phenotypically confirmed as carbapenemase producers using the modified carbapenem inactivation method test. The isolates were resistant to a wide range of antibiotics including β-lactams (48.1%–100%), two fluoroquinolones (77.8%–88.9%), tigecycline and minocycline (70.4% each), and sulfamethoxazole-trimethoprim (55.6%). Whole-genome sequencing was conducted on all carbapenem-resistant strains, uncovering bla_IMP in eight isolates, comprising seven with bla_IMP-1 and one with bla_IMP-11, alongside multiple antimicrobial resistance determinants. Importantly, the phylogenomic comparison with international S. marcescens isolates revealed genetic relatedness and potential cross-border transmission events.

Conclusions

Our findings underscore the importance of enhanced surveillance and infection control measures to mitigate the dissemination of multidrug-resistant pathogens, emphasizing the need for international collaboration and coordinated efforts to address antimicrobial resistance on a global scale.

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.