揭示4型牛疱疹病毒(BHV-4)在奶牛生殖障碍中的作用:来自中国多方面研究的见解

IF 3.5 2区 农林科学 Q2 INFECTIOUS DISEASES
Lingling Zhu, Xinjun Dai, Yuxin Lai, Zhigang Guo, Yiping Gu, Jianwei Zhang, Hongyu Wang, Guangjun Chang, Huochun Yao, Zihao Pan
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引用次数: 0

摘要

牛疱疹病毒4型(BHV-4)属于γ -疱疹病毒亚家族,其与奶牛生殖障碍的关系存在争议。在这项研究中,收集了中国8个省份的386头奶牛的全血和生殖拭子样本。采用ELISA法检测血清中BHV-4抗体水平,采用实时荧光定量PCR (qPCR)检测样品中BHV-4、BHV-1、牛病毒性腹泻病毒i型(BVDV-1)和牛病毒性腹泻病毒ii型(BVDV-2)的DNA。此外,通过qPCR检测奶牛和死胎各组织中BHV-4的DNA含量。通过农户访谈收集参与奶牛的育种信息,并对检测结果与育种信息进行相关性分析。结果显示BHV-4抗体阳性率为57.8%,核酸阳性率为36.3%。卡方分析显示BHV-4和BHV-1感染之间存在相关性。在几种与生殖障碍相关的病原体中,只有BHV-4具有显著相关性,而BHV-1、BVDV-1和BVDV-2没有相关性。此外,该研究还揭示了奶牛和死胎生殖道中BHV-4 DNA的升高。组织病理学切片显示粘膜损伤阴道和子宫组织的死产胎儿,这一现象以前没有报道过。总之,我们的研究为BHV-4与生殖疾病之间的相关性提供了新的见解,并提供了支持BHV-4垂直传播的新证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Unveiling the Role of Bovine Herpesvirus Type 4 (BHV-4) in Dairy Cow Reproductive Disorders: Insights From a Multifaceted Study in China

Unveiling the Role of Bovine Herpesvirus Type 4 (BHV-4) in Dairy Cow Reproductive Disorders: Insights From a Multifaceted Study in China

Bovine herpesvirus type-4 (BHV-4) belongs to the gamma-herpesvirus subfamily, and its association with reproductive disorders in dairy cows is controversial. In this study, 386 whole blood and reproductive swab samples from dairy cows across eight provinces in China were collected. BHV-4 antibody levels in the serum were determined via ELISA, while real-time fluorescent quantitative PCR (qPCR) was employed to detect the DNA of BHV-4, BHV-1, bovine viral diarrhea virus type-I (BVDV-1), and bovine viral diarrhea virus type-II (BVDV-2) in the samples. Additionally, the DNA content of BHV-4 in various tissues of cows and stillborn fetuses was assessed via qPCR. Breeding information for the participating cows was gathered through farmer interviews, and correlation analyses were conducted between the detection results and breeding information. The findings revealed a BHV-4 antibody positive rate of 57.8% and a nucleic acid positive rate of 36.3%. Chi-square analysis revealed a correlation between BHV-4 and BHV-1 infections. Among several pathogens associated with reproductive disorders, only BHV-4 was significantly correlated, whereas BHV-1, BVDV-1, and BVDV-2 were not correlated. Furthermore, the study revealed elevated BHV-4 DNA in the reproductive tracts of cows and stillborn fetuses. Histopathological sections revealed mucosal damage in the vaginal and uterine tissues of stillborn fetuses, a phenomenon not previously reported. In summary, our study provides novel insight into the correlation between BHV-4 and reproductive disorders and presents new evidence that supports the vertical transmission of BHV-4.

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来源期刊
Transboundary and Emerging Diseases
Transboundary and Emerging Diseases 农林科学-传染病学
CiteScore
8.90
自引率
9.30%
发文量
350
审稿时长
1 months
期刊介绍: Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions): Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread. Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope. Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies. Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies). Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.
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