日本首次分离到蝙蝠杰龙病毒并鉴定

IF 3.5 2区 农林科学 Q2 INFECTIOUS DISEASES
Sho Sata, Isshu Kojima, Mana Esaki, Kimitake Funakoshi, Masahiro Kajihara, Shinji Hirano, Shin Murakami, Kazuo Miyazaki, Makoto Ozawa, Kosuke Okuya
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引用次数: 0

摘要

蝙蝠是几种副粘病毒的天然宿主,这引起了人们对这些病毒可能引起跨物种感染的担忧。本研究从日本鹿儿岛县东部弯翅蝠(Miniopterus fuliginosus)和远东肌炎蝠(myotis bombinus)的口腔拭子样本中分离到了两种属于副粘病毒科的jeilong病毒。值得注意的是,这是日本首次报道分离出蝙蝠副粘病毒。基因组分析显示鹿鹿岛分离株(PMV/Bat35和PMV/Bat111)与先前于2016年在韩国从Schreiber弯翼蝙蝠(Miniopterus schreibersii)中分离到的jeilong病毒B16-40具有高度同源性。PMV/Bat35在来自不同动物物种的一系列细胞系中感染和复制,尽管合胞体形成水平在细胞系之间有所不同。动物实验显示,经鼻内接种PMV/Bat35的叙利亚仓鼠没有表现出临床症状或显著体重减轻。然而,在感染后2天和5天,在叙利亚仓鼠的肺和气管中检测到病毒基因。重要的是,抗PMV/Bat35的中和抗体在14 dpi的仓鼠中产生。这些结果表明,蝙蝠杰龙病毒可以跨越物种屏障。我们的研究结果强调了持续监测和表征蝙蝠种群中传播的病毒以评估人畜共患病暴发风险的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The First Isolation and Characterization of Bat Jeilongviruses in Japan

The First Isolation and Characterization of Bat Jeilongviruses in Japan

Bats represent natural reservoirs of several paramyxoviruses, raising concerns about the potential for these viruses to cause cross-species infections. In this study, we isolated two jeilongviruses belonging to the family Paramyxoviridae from oral swab samples of the Eastern bent-wing bat (Miniopterus fuliginosus) and Far Eastern myotis bat (Myotis bombinus) in Kagoshima Prefecture, Japan. Notably, this is the first report isolating bat paramyxoviruses in Japan. Genomic analyses revealed a high identity between Kagoshima isolates (PMV/Bat35 and PMV/Bat111) and jeilongvirus B16-40, previously isolated from a Schreiber’s bent-wing bat (Miniopterus schreibersii) in South Korea in 2016. PMV/Bat35 infected and replicated in a range of cell lines derived from different animal species, although the level of syncytium formation varied among cell lines. Animal experiments revealed that Syrian hamsters inoculated intranasally with PMV/Bat35 did not exhibit clinical symptoms or significant weight loss. Nevertheless, viral genes were detected in the lungs and tracheas of Syrian hamsters on 2- and 5-day postinfection (dpi). Importantly, neutralizing antibodies against PMV/Bat35 developed in hamsters on 14 dpi. These results suggest that bat jeilongviruses can cross the species barriers. Our findings highlight the critical importance of ongoing monitoring and characterization of viruses circulating in bat populations to assess the risk of zoonotic outbreaks.

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来源期刊
Transboundary and Emerging Diseases
Transboundary and Emerging Diseases 农林科学-传染病学
CiteScore
8.90
自引率
9.30%
发文量
350
审稿时长
1 months
期刊介绍: Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions): Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread. Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope. Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies. Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies). Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.
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