{"title":"基于半透聚间苯二胺膜和固定化乳酸氧化酶的安培生物传感器高精度测定血清中l-乳酸","authors":"Kseniia Berketa, Anhelina Buzhak, Yevhen Vakhovskyi, Daryna Mruga, Andrii Sverstiuk, Olha Soldatkina, Olha Lyubovych, Olga Marchuk, Serhii Dzyadevych, Oleksandr Soldatkin","doi":"10.1002/elan.12011","DOIUrl":null,"url":null,"abstract":"<p>This article presents the development and optimization of a biosensor for the accurate and rapid determination of <span>l</span>-lactate concentration in human serum samples. The biosensor uses lactate oxidase immobilized in a bovine serum albumin matrix on a platinum transducer, modified with a semipermeable <i>m</i>-polyphenylenediamine (PPD) membrane. The PPD membrane minimizes influence from solution parameters like ionic strength and protein content, as well as interferents in blood serum on an analysis result. The biosensor offers a broad linear range (7–1000 μM), low detection limit (7 μM), and short analysis time (8 min), making it suitable for real serum samples measurement. Results showed high correlation with reference methods, with a correlation coefficient of 0.982 in comparison with standard analysis method. Stability tests indicated an RSD of 2.9% and 6.7% for model and real samples analysis, with functionality sustained over 5 days. This biosensor is promising for clinical diagnostics due to its speed, accuracy, and stability in measuring <span>l</span>-lactate.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 1","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Amperometric Biosensor Based on a Semipermeable Poly-Meta-Phenylenediamine Membrane and Immobilized Lactate Oxidase for Highly Accurate l-Lactate Determination in Blood Serum\",\"authors\":\"Kseniia Berketa, Anhelina Buzhak, Yevhen Vakhovskyi, Daryna Mruga, Andrii Sverstiuk, Olha Soldatkina, Olha Lyubovych, Olga Marchuk, Serhii Dzyadevych, Oleksandr Soldatkin\",\"doi\":\"10.1002/elan.12011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This article presents the development and optimization of a biosensor for the accurate and rapid determination of <span>l</span>-lactate concentration in human serum samples. The biosensor uses lactate oxidase immobilized in a bovine serum albumin matrix on a platinum transducer, modified with a semipermeable <i>m</i>-polyphenylenediamine (PPD) membrane. The PPD membrane minimizes influence from solution parameters like ionic strength and protein content, as well as interferents in blood serum on an analysis result. The biosensor offers a broad linear range (7–1000 μM), low detection limit (7 μM), and short analysis time (8 min), making it suitable for real serum samples measurement. Results showed high correlation with reference methods, with a correlation coefficient of 0.982 in comparison with standard analysis method. Stability tests indicated an RSD of 2.9% and 6.7% for model and real samples analysis, with functionality sustained over 5 days. This biosensor is promising for clinical diagnostics due to its speed, accuracy, and stability in measuring <span>l</span>-lactate.</p>\",\"PeriodicalId\":162,\"journal\":{\"name\":\"Electroanalysis\",\"volume\":\"37 1\",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-01-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Electroanalysis\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/elan.12011\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electroanalysis","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/elan.12011","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Amperometric Biosensor Based on a Semipermeable Poly-Meta-Phenylenediamine Membrane and Immobilized Lactate Oxidase for Highly Accurate l-Lactate Determination in Blood Serum
This article presents the development and optimization of a biosensor for the accurate and rapid determination of l-lactate concentration in human serum samples. The biosensor uses lactate oxidase immobilized in a bovine serum albumin matrix on a platinum transducer, modified with a semipermeable m-polyphenylenediamine (PPD) membrane. The PPD membrane minimizes influence from solution parameters like ionic strength and protein content, as well as interferents in blood serum on an analysis result. The biosensor offers a broad linear range (7–1000 μM), low detection limit (7 μM), and short analysis time (8 min), making it suitable for real serum samples measurement. Results showed high correlation with reference methods, with a correlation coefficient of 0.982 in comparison with standard analysis method. Stability tests indicated an RSD of 2.9% and 6.7% for model and real samples analysis, with functionality sustained over 5 days. This biosensor is promising for clinical diagnostics due to its speed, accuracy, and stability in measuring l-lactate.
期刊介绍：
Electroanalysis is an international, peer-reviewed journal covering all branches of electroanalytical chemistry, including both fundamental and application papers as well as reviews dealing with new electrochemical sensors and biosensors, nanobioelectronics devices, analytical voltammetry, potentiometry, new electrochemical detection schemes based on novel nanomaterials, fuel cells and biofuel cells, and important practical applications.
Serving as a vital communication link between the research labs and the field, Electroanalysis helps you to quickly adapt the latest innovations into practical clinical, environmental, food analysis, industrial and energy-related applications. Electroanalysis provides the most comprehensive coverage of the field and is the number one source for information on electroanalytical chemistry, electrochemical sensors and biosensors and fuel/biofuel cells.
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