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IF 3 3区医学 Q1 OPHTHALMOLOGY

Acta Ophthalmologica Pub Date : 2025-01-19 DOI:10.1111/aos.16950

Hamid Goodarzi, Boda Om, May Griffith, Christos Boutopoulos

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引用次数: 0

摘要

目的：角膜穿孔是一种可导致失明的医疗急症。治疗方法包括氰基丙烯酸酯或纤维蛋白胶，两者都有副作用，包括细胞毒性。以明胶甲基丙烯酰为基础的生物材料是这些粘合剂的替代品[1, 2]。获得光学清晰度和光滑的表面完整性对于治疗后的角膜伤口恢复视力至关重要。方法：目前，角膜生物材料的输送采用标准注射器系统，该系统缺乏重建角膜形状的准确性。因此，我们研究了使用 Boutopoulos 实验室开发的激光生物打印技术[3]来实现精确的原位角膜伤口修复。我们使用了一种由甲基丙烯酰明胶（GelMa）、丙烯酸羟乙酯（HEA）和苯基-2,4,6-三甲基苯甲酰膦酸锂（LAP）组成的光交联墨水作为光引发剂。结果：使用 230 μJ 的激光能量、每分钟 20 微升的流速和 37°C 的温度生成纳升体积的单个液滴，从而优化了打印性能。流变学、光学表征和爆破压力测量评估了其密封角膜穿孔的潜力。结果表明，打印 LiQD 角膜的水凝胶储存模量为 1.31 ± 0.31 KPa，用于密封尸体猪眼睛的全厚度角膜穿孔时，爆破压力为 38 ± 6 mmHg。观察到的光学清晰度与原生角膜相似（透光率：93.12 ± 1.02 印刷值 vs 92.61 ± 1.50）。OCT 结果表明，LIST 技术有可能填充角膜伤口，并重建受伤角膜的自然弧度。结论：总之，我们开发出了一种精确的打印系统，用于向伤口输送粘性角膜再生生物材料，并对其进行了表征。参考文献 1.Sharifi, S., et al., 2021.6(11): p. 3947-3961. 2.Barroso, I.A., et al., 2022.9(2). 3.Ebrahimi Orimi, H., et al., Sci Rep, 2020.10(1): p. 9730.

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In-situ printing of gelma-based hydrogels for cornea regeneration

Aims/Purpose: Corneal perforation is a medical emergency that can lead to blindness. Treatment options encompass cyanoacrylate or fibrin glue, both linked to side effects, including cytotoxicity. Gelatin methacryloyl-based biomaterials offer an alternative to these adhesives [1, 2]. Achieving optical clarity and smooth surface integrity is crucial for the restoration of vision in treated corneal wounds.

Methods: Currently, the delivery of corneal biomaterials utilizes standard syringe systems, which lack the accuracy to reconstruct the cornea's shape. Therefore, the use of a laser bioprinting technology, developed in the Boutopoulos lab [3], for achieving precise in-situ corneal wound repair was examined. We used a photocrosslinkable ink comprising Gelatin Methacryloyl (GelMa), hydroxyethyl acrylate (HEA), and Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as a photoinitiator.

Results: Printability was optimized by generating nanoliter-volume individual droplets using 230 μJ laser energy, a flow rate 20 microliter per minute, and a temperature of 37°C. Rheology, optical characterization, and bursting pressure measurements assessed its potential to seal corneal perforations. Results indicated a hydrogel storage modulus of 1.31 ± 0.31 KPa for printed LiQD cornea and a bursting pressure of 38 ± 6 mmHg when used to seal full thickness cornea perforation in cadaveric pig eyes. Optical clarity akin to the native cornea was observed (%light transmission: 93.12 ± 1.02 printed vs 92.61 ± 1.50). The OCT results showed that the LIST technique could potentially fill the corneal wounds and reconstruct the natural curvature of the wounded cornea.

Conclusions: In conclusion, a precise printing system for delivering adhesive corneal regenerative biomaterials to wounds was developed and characterized.

References

1. Sharifi, S., et al., 2021. 6(11): p. 3947-3961.

2. Barroso, I.A., et al., 2022. 9(2).

3. Ebrahimi Orimi, H., et al., Sci Rep, 2020. 10(1): p. 9730.

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来源期刊

Acta Ophthalmologica 医学-眼科学

CiteScore

7.60

自引率

5.90%

发文量

433

审稿时长

6 months

期刊介绍： Acta Ophthalmologica is published on behalf of the Acta Ophthalmologica Scandinavica Foundation and is the official scientific publication of the following societies: The Danish Ophthalmological Society, The Finnish Ophthalmological Society, The Icelandic Ophthalmological Society, The Norwegian Ophthalmological Society and The Swedish Ophthalmological Society, and also the European Association for Vision and Eye Research (EVER). Acta Ophthalmologica publishes clinical and experimental original articles, reviews, editorials, educational photo essays (Diagnosis and Therapy in Ophthalmology), case reports and case series, letters to the editor and doctoral theses.