Reet Mändar, Imbi Smidt, Tiiu Rööp, Dagmar Hoidmets, Kalle Kilk, Mihkel Zilmer, Kristo Ausmees, Andres Salumets
{"title":"有益男性生殖道健康的益生菌的开发：体外实验和健康志愿者临床试验","authors":"Reet Mändar, Imbi Smidt, Tiiu Rööp, Dagmar Hoidmets, Kalle Kilk, Mihkel Zilmer, Kristo Ausmees, Andres Salumets","doi":"10.1155/and/7000428","DOIUrl":null,"url":null,"abstract":"<div>\n <p><b>Background:</b> Health of the male urogenital tract is closely related to the microbial communities in this anatomical area. Disturbed microbial communities can be balanced with the help of carefully selected strains in which the presence of both general and specific probiotic effects has been described.</p>\n <p><b>Objectives:</b> We aimed to develop novel probiotic that could be used to support male reproductive health.</p>\n <p><b>Materials and Methods:</b> Suitable lactobacilli strains were screened from culture collection in order to find the most suitable strains for male genital tract. Clinical trial included 10 volunteer men who considered themselves healthy. They consumed enteric-coated oral capsules containing four lactobacilli strains. Blood markers and questionnaire data were recorded during the consumption period.</p>\n <p><b>Results:</b> The selected lactobacilli strains (two <i>L. crispatus</i>, one <i>L. gasseri</i>, one <i>L. ruminis</i>) expressed good tolerance to high pH and antagonistic activity against prostatitis-associated opportunistic bacteria. Metabolic profiling of the selected strains revealed beneficial properties for both reproductive and general health. The probiotic capsules appeared to be generally well tolerated during 1-week period. No serious adverse events and no significant changes in haemogram were observed.</p>\n <p><b>Conclusions:</b> The study confirmed that certain probiotic properties in lactobacilli are highly strain-specific, and we succeeded to select a set of strains that can be suitable for supporting male reproductive health. Our study suggested that daily oral administration of the selected strains at the dose level of billion CFU/dose during 1 week is well tolerated by healthy middle-aged men. Therefore, these strains seem to be promising candidates for the development of novel evidence-based well-focused probiotics to target male urogenital tract disorders. Their effectiveness must be confirmed in clinical trials on diseased patients.</p>\n </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/7000428","citationCount":"0","resultStr":"{\"title\":\"Development of Probiotics for Male Reproductive Tract Health: In Vitro Experiments and Clinical Trial on Healthy Volunteers\",\"authors\":\"Reet Mändar, Imbi Smidt, Tiiu Rööp, Dagmar Hoidmets, Kalle Kilk, Mihkel Zilmer, Kristo Ausmees, Andres Salumets\",\"doi\":\"10.1155/and/7000428\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n <p><b>Background:</b> Health of the male urogenital tract is closely related to the microbial communities in this anatomical area. Disturbed microbial communities can be balanced with the help of carefully selected strains in which the presence of both general and specific probiotic effects has been described.</p>\\n <p><b>Objectives:</b> We aimed to develop novel probiotic that could be used to support male reproductive health.</p>\\n <p><b>Materials and Methods:</b> Suitable lactobacilli strains were screened from culture collection in order to find the most suitable strains for male genital tract. Clinical trial included 10 volunteer men who considered themselves healthy. They consumed enteric-coated oral capsules containing four lactobacilli strains. Blood markers and questionnaire data were recorded during the consumption period.</p>\\n <p><b>Results:</b> The selected lactobacilli strains (two <i>L. crispatus</i>, one <i>L. gasseri</i>, one <i>L. ruminis</i>) expressed good tolerance to high pH and antagonistic activity against prostatitis-associated opportunistic bacteria. Metabolic profiling of the selected strains revealed beneficial properties for both reproductive and general health. The probiotic capsules appeared to be generally well tolerated during 1-week period. No serious adverse events and no significant changes in haemogram were observed.</p>\\n <p><b>Conclusions:</b> The study confirmed that certain probiotic properties in lactobacilli are highly strain-specific, and we succeeded to select a set of strains that can be suitable for supporting male reproductive health. Our study suggested that daily oral administration of the selected strains at the dose level of billion CFU/dose during 1 week is well tolerated by healthy middle-aged men. Therefore, these strains seem to be promising candidates for the development of novel evidence-based well-focused probiotics to target male urogenital tract disorders. 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Development of Probiotics for Male Reproductive Tract Health: In Vitro Experiments and Clinical Trial on Healthy Volunteers
Background: Health of the male urogenital tract is closely related to the microbial communities in this anatomical area. Disturbed microbial communities can be balanced with the help of carefully selected strains in which the presence of both general and specific probiotic effects has been described.
Objectives: We aimed to develop novel probiotic that could be used to support male reproductive health.
Materials and Methods: Suitable lactobacilli strains were screened from culture collection in order to find the most suitable strains for male genital tract. Clinical trial included 10 volunteer men who considered themselves healthy. They consumed enteric-coated oral capsules containing four lactobacilli strains. Blood markers and questionnaire data were recorded during the consumption period.
Results: The selected lactobacilli strains (two L. crispatus, one L. gasseri, one L. ruminis) expressed good tolerance to high pH and antagonistic activity against prostatitis-associated opportunistic bacteria. Metabolic profiling of the selected strains revealed beneficial properties for both reproductive and general health. The probiotic capsules appeared to be generally well tolerated during 1-week period. No serious adverse events and no significant changes in haemogram were observed.
Conclusions: The study confirmed that certain probiotic properties in lactobacilli are highly strain-specific, and we succeeded to select a set of strains that can be suitable for supporting male reproductive health. Our study suggested that daily oral administration of the selected strains at the dose level of billion CFU/dose during 1 week is well tolerated by healthy middle-aged men. Therefore, these strains seem to be promising candidates for the development of novel evidence-based well-focused probiotics to target male urogenital tract disorders. Their effectiveness must be confirmed in clinical trials on diseased patients.
期刊介绍：
Andrologia provides an international forum for original papers on the current clinical, morphological, biochemical, and experimental status of organic male infertility and sexual disorders in men. The articles inform on the whole process of advances in andrology (including the aging male), from fundamental research to therapeutic developments worldwide. First published in 1969 and the first international journal of andrology, it is a well established journal in this expanding area of reproductive medicine.
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