茶蜂花粉与细菌和酶协同发酵对中华蜜蜂生长和肠道健康的影响

IF 5.8 Q1 MICROBIOLOGY
Panpan Xue , Le Xu , Yakai Tian , Mingkui Lv , Pingping Fang , Kun Dong , Qiuye Lin , Zhenhui Cao
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引用次数: 0

摘要

虽然乳酸菌(LAB)发酵饲料对养殖动物的健康益处是公认的,但它对蜜蜂(特别是蜜蜂)的潜在益处在很大程度上仍未被探索。本研究旨在优化茶蜂花粉的酶解工艺,采用一种由酸性纤维素酶和果胶酶组成的复合酶,然后用罗伊氏乳酸杆菌LP4发酵。随后用该方法处理的茶蜂花粉饲喂蜜蜂工蜂。在发酵茶蜂花粉的最佳工艺条件下,发酵茶蜂花粉的pH值为4.41,蛋白质含量为27.75%,LAB活菌数为2.31×10 (9 CFU/g)。未检出霉菌、酵母菌及病原菌。与未发酵花粉相比,饲喂含有罗伊氏乳杆菌LP4的发酵茶花粉的工蜂在第15天的存活率显著提高了24.34%。此外,乳杆菌和双歧杆菌的相对丰度升高,肠球菌和拟杆菌的相对丰度降低。同时,Abaecin、PPO、Defensin、Vg等免疫相关基因的相对表达量显著上调。研究结果为应用发酵饲料提高中华蜜蜂种群的健康水平和促进中国养蜂业的可持续发展提供了科学依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana

Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana
While the health benefits of lactic acid bacteria (LAB)-fermented feed on farmed animals are well-established, its potential benefits for honeybees, specifically Apis cerana cerana, remain largely unexplored. The present study aimed to optimize an enzymatic hydrolysis process for tea bee pollen, employing a complex enzyme comprising acid cellulase and pectinase, followed by fermentation with Limosilactobacillus reuteri LP4. A. c. cerana workers were subsequently fed tea bee pollen processed with this optimized method. Under the optimal processing condition of fermented tea bee pollen, the pH value was 4.41, the protein content was 27.75 %, and the viable count of LAB was 2.31×10⁹ CFU/g. No molds and yeasts as well as pathogens were detected. Compared to the unfermented pollen, honey bee workers administrated with fermented tea pollen with L. reuteri LP4 showed significantly increased survival rate by 24.34 % on day 15. Moreover, the relative abundances of Lactobacillus and Bifidobacterium were elevated, while those of Enterococcus and Bacteroides were diminished. Concurrently, the relative expression levels of immune-related genes including Abaecin, PPO, Defensin, and Vg were significantly upregulated. These findings provide a scientific foundation for application of fermented feeds to enhance the health of A. c. cerana populations and contribute to the sustainable development of apiculture in China.
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来源期刊
Current Research in Microbial Sciences
Current Research in Microbial Sciences Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
7.90
自引率
0.00%
发文量
81
审稿时长
66 days
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