Nicole S. Lameirinhas , João P.F. Carvalho , Maria C. Teixeira , Jorge L. Luís , Asiyah Esmail , Ricardo J.B. Pinto , Helena Oliveira , Filomena Freitas , José M. Oliveira , Carla Vilela , Armando J.D. Silvestre , Carmen S.R. Freire
{"title":"纳米复合水凝胶为基础的生物墨水,由富含焦点的多糖和纳米纤维素纤维组成,用于3d生物打印应用","authors":"Nicole S. Lameirinhas , João P.F. Carvalho , Maria C. Teixeira , Jorge L. Luís , Asiyah Esmail , Ricardo J.B. Pinto , Helena Oliveira , Filomena Freitas , José M. Oliveira , Carla Vilela , Armando J.D. Silvestre , Carmen S.R. Freire","doi":"10.1016/j.bprint.2024.e00382","DOIUrl":null,"url":null,"abstract":"<div><div>Hydrogels are the most common type of bioinks, yet, finding adequate biomaterials to develop suitable bioinks for 3D bioprinting remains challenging. Herein, innovative hydrogel bioinks were developed by combining nanofibrillated cellulose (NFC) with a fucose-rich polysaccharide, FucoPol (FP), still unexplored for 3D bioprinting. NFC/FP bioinks with different mass proportions, namely 1:1, 2:1, 3:1 and 4:1, were prepared and denominated as NFC1FP, NFC2FP, NFC3FP and NFC4FP. A formulation without NFC was also prepared for comparison purposes (NFC0FP). The rheological properties of the bioinks were enhanced by the addition of NFC, as evidenced by the increase in shear viscosity from 1.39 ± 0.03 Pa s (NFC0FP) to 2933.7 ± 137.9 Pa s (ink NFC4FP) and by the 3D printing of complex structures with high shape fidelity (<em>Pr</em> ≈ 0.9). The stability and mechanical properties of the crosslinked hydrogels were also improved, with Young’s modulus increasing from 0.12 ± 0.04 MPa (NFC0FP) to 2.45 ± 0.06 MPa (NFC4FP). The successful 3D bioprinting of both A375 (melanoma) and HaCaT (keratinocyte) cell-laden bioinks translated into elevated cell viabilities (above 88 %) up to 21 days post-bioprinting. These results highlight the potential and versatility of NFC/FP bioinks for the bioprinting of 3D skin tissue analogues for biomedical applications.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"45 ","pages":"Article e00382"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Nanocomposite hydrogel-based bioinks composed of a fucose-rich polysaccharide and nanocellulose fibers for 3D-bioprinting applications\",\"authors\":\"Nicole S. Lameirinhas , João P.F. Carvalho , Maria C. Teixeira , Jorge L. Luís , Asiyah Esmail , Ricardo J.B. Pinto , Helena Oliveira , Filomena Freitas , José M. Oliveira , Carla Vilela , Armando J.D. Silvestre , Carmen S.R. 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引用次数: 0
摘要
水凝胶是最常见的生物墨水类型,然而,寻找足够的生物材料来开发适合3D生物打印的生物墨水仍然具有挑战性。在这里,创新的水凝胶生物墨水是通过将纳米纤维化纤维素(NFC)与富含焦点的多糖——岩藻酚(FP)结合而开发出来的,这种多糖尚未被用于生物3D打印。制备了质量比例分别为1:1、2:1、3:1和4:1的NFC/FP生物墨水,分别命名为NFC1FP、NFC2FP、NFC3FP和NFC4FP。同时制备了不含NFC的配方(NFC0FP)进行比较。NFC的加入增强了生物墨水的流变性能,剪切粘度从1.39±0.03 Pa s (NFC0FP)增加到2933.7±137.9 Pa s (NFC4FP), 3D打印复杂结构具有较高的形状保真度(Pr≈0.9)。交联水凝胶的稳定性和力学性能也得到了改善,杨氏模量从0.12±0.04 MPa (NFC0FP)增加到2.45±0.06 MPa (NFC4FP)。A375(黑色素瘤)和HaCaT(角质形成细胞)细胞负载生物墨水的成功3D生物打印在生物打印后21天内转化为更高的细胞存活率(超过88%)。这些结果突出了NFC/FP生物墨水用于生物医学应用的3D皮肤组织类似物生物打印的潜力和多功能性。
Nanocomposite hydrogel-based bioinks composed of a fucose-rich polysaccharide and nanocellulose fibers for 3D-bioprinting applications
Hydrogels are the most common type of bioinks, yet, finding adequate biomaterials to develop suitable bioinks for 3D bioprinting remains challenging. Herein, innovative hydrogel bioinks were developed by combining nanofibrillated cellulose (NFC) with a fucose-rich polysaccharide, FucoPol (FP), still unexplored for 3D bioprinting. NFC/FP bioinks with different mass proportions, namely 1:1, 2:1, 3:1 and 4:1, were prepared and denominated as NFC1FP, NFC2FP, NFC3FP and NFC4FP. A formulation without NFC was also prepared for comparison purposes (NFC0FP). The rheological properties of the bioinks were enhanced by the addition of NFC, as evidenced by the increase in shear viscosity from 1.39 ± 0.03 Pa s (NFC0FP) to 2933.7 ± 137.9 Pa s (ink NFC4FP) and by the 3D printing of complex structures with high shape fidelity (Pr ≈ 0.9). The stability and mechanical properties of the crosslinked hydrogels were also improved, with Young’s modulus increasing from 0.12 ± 0.04 MPa (NFC0FP) to 2.45 ± 0.06 MPa (NFC4FP). The successful 3D bioprinting of both A375 (melanoma) and HaCaT (keratinocyte) cell-laden bioinks translated into elevated cell viabilities (above 88 %) up to 21 days post-bioprinting. These results highlight the potential and versatility of NFC/FP bioinks for the bioprinting of 3D skin tissue analogues for biomedical applications.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.