高分辨率3D生物打印的进展：探索技术趋势，生物墨水和实现的分辨率

Q1 Computer Science

Bioprinting Pub Date : 2024-12-01 DOI:10.1016/j.bprint.2024.e00376

Luca Guida, Marco Cavallaro, Marinella Levi

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引用次数: 0

摘要

3D生物打印是一个快速发展的领域，在技术、材料和策略方面都取得了重大进展。它能够生产活组织和复杂的生物结构，为再生医学、药物测试和个性化医疗提供了巨大的潜力。取得了显著进展，特别是在开发模拟生理环境和促进组织生长的材料方面。然而，要制造复杂的、大规模的、异细胞的结构体，还有很多工作要做。高分辨率印刷和技术发展对实现这一目标至关重要。尽管这一主题具有重要意义，但文献缺乏全面的综述，重点是分析生物打印中实现的分辨率和量化指标。此外，以前的工作没有检查所有最相关的技术，批判性地强调技术优势，如分辨率和识别限制，如结构的特征维度。本文综述了生物3D打印的各个方面，重点介绍了最常用的技术，包括基于挤压的生物打印、还原光聚合、喷墨、激光诱导正向转移和双光子聚合。此外，它还检查了与这些技术兼容的生物材料和交联策略。主要的焦点是分辨率表征的重要性，评估技术优势，并从文献中总结常见的度量标准。这篇综述评估了不同生物打印方法的分辨率，将这些数据与每种技术的适用性和局限性联系起来，因为单独的分辨率不足以产生功能结构。已经报道了一些克服某些技术典型分辨率限制的策略。在这样做的过程中，重点放在旨在生物模式和生产组织工程支架的工作上，因此涉及到活细胞的使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Advancements in high-resolution 3D bioprinting: Exploring technological trends, bioinks and achieved resolutions

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Advancements in high-resolution 3D bioprinting: Exploring technological trends, bioinks and achieved resolutions

3D bioprinting is a rapidly evolving field that has seen significant advancements in technologies, materials, and strategies. It enables the production of living tissues and complex biological structures, offering great potential for regenerative medicine, drug testing, and personalized medical treatments.

Notable progress has been done, particularly in developing materials that mimic the physiological environment and promote tissue growth. However, much work is still needed to fabricate complex, large-scale, heterocellular constructs. High-resolution printing and technological development are crucial to this goal.

Despite the significance of this topic, the literature lacks comprehensive reviews focused on analyzing the achieved resolution and metrics for its quantification in bioprinting. Additionally, no previous work examines all the most relevant technologies, critically highlighting technological advantages such as resolution and identifying limitations like the characteristic dimensions of constructs.

This review examines various aspects of 3D bioprinting, focusing on the most commonly used technologies, including Extrusion-Based Bioprinting, Vat Photopolymerization, Inkjet, Laser-Induced Forward Transfer, and Two-Photon Polymerization. Additionally, it examines the biomaterials and crosslinking strategies compatible with each of these technologies.

The primary focus is on the importance of resolution characterization, assessing technical advantages, and summarizing common metrics from the literature. The review evaluates the resolutions achieved across different bioprinting methods, correlating such data with the applicability and limitations of each technology, as resolution alone is not sufficient for producing functional structures. Some strategies to overcome typical resolution limits of some technologies have been reported.

In doing so, the focus is kept on works aimed at biological patterning and producing scaffolds for tissue engineering, therefore involving the use of live cells.

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来源期刊

Bioprinting Computer Science-Computer Science Applications

CiteScore

11.50

自引率

0.00%

发文量

审稿时长

68 days

期刊介绍： Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.