人羊膜间充质干细胞联合头皮针刺可减轻脑瘫大鼠脑损伤

IF 2 Q3 NEUROSCIENCES

IBRO Neuroscience Reports Pub Date : 2025-01-02 DOI:10.1016/j.ibneur.2024.12.015

Yu Zhou , Xu-Huan Li , Lu-Na He , Li-Na Wang , Jing Zang , Dong-Ming Wang , Jing Gao , Xue-feng Yu

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引用次数: 0

摘要

背景：脑瘫（CP）是儿童身体残疾的常见原因，通常由缺氧缺血性脑病引起，目前的治疗方法往往不能解决潜在的病理生理问题。本研究旨在探讨人羊膜间充质干细胞（hAMSCs）联合头皮针刺对CP模型大鼠的潜在协同作用。方法雄性sd大鼠20只，随机分为Sham组、CP组、hAMSCs组和hAMSCs+头皮针刺组（hAMSCs+AP）。结扎左颈总动脉造模。经尾静脉注射hAMSCs，然后头皮针刺百会穴（GV20）和曲滨穴（GB7）。使用Bederson评分评估神经行为功能，并使用苏木精和伊红（H&；E）染色，TUNEL染色和RT-qPCR分析脑组织中凋亡相关基因。结果CP组表现出明显的神经行为缺陷和细胞凋亡增加。hAMSCs和hAMSCs+AP治疗均能改善神经行为功能，减少细胞凋亡。联合治疗进一步降低细胞凋亡水平，使Bax、Caspase 9、Caspase 3 mRNA表达正常化，减轻组织损伤。结论hAMSCs联合头皮针刺治疗CP是一种很有前景的治疗方法，可能通过调节细胞凋亡来减轻脑损伤。需要进一步的研究来阐明详细的机制，评估临床可行性和安全性。

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Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy

Background

Cerebral palsy (CP) is a prevalent cause of physical disability in children, often resulting from hypoxic-ischemic encephalopathy, with current therapies often failing to address the underlying pathophysiology. This study aimed to investigate the potential synergistic effects of human amnion-derived mesenchymal stem cells (hAMSCs) combined with scalp acupuncture in a rat model of CP.

Methods

Twenty male Sprague-Dawley rats were randomly divided into four groups: Sham, CP, hAMSCs, and hAMSCs+scalp acupuncture (hAMSCs+AP). The CP model was induced via left common carotid artery ligation. hAMSCs were administered through tail vein injection, followed by scalp acupuncture at Baihui (GV20) and Qubin (GB7) points. Neurobehavioral function was assessed using the Bederson score, and brain tissues were analyzed using hematoxylin and eosin (H&E) staining, TUNEL staining, and RT-qPCR for apoptosis-related genes.

Results

The CP group exhibited significant neurobehavioral deficits and increased apoptosis. Both hAMSCs and hAMSCs+AP treatments improved neurobehavioral function and reduced apoptosis. The combination therapy further decreased apoptosis levels, normalized mRNA expression of Bax, Caspase 9, and Caspase 3, and alleviated histological damage.

Conclusions

The combination of hAMSCs and scalp acupuncture provides a promising treatment for CP, potentially alleviating brain damage through apoptosis regulation. Further studies are required to elucidate the detailed mechanisms and assess clinical feasibility and safety.

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