无硬件自适应光学的时空光学相干断层成像（stock - t）视网膜神经节细胞和光感受器成像

IF 6.6 2区医学 Q1 ENGINEERING, BIOMEDICAL

Biocybernetics and Biomedical Engineering Pub Date : 2025-01-01 DOI:10.1016/j.bbe.2025.01.001

Marta Mikuła-Zdańkowska , Dawid Borycki , Piotr Węgrzyn , Karolis Adomavičius , Egidijus Auksorius , Maciej Wojtkowski

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引用次数: 0

摘要

我们展示了一个实验性的时空光学相干断层扫描（stock - t）系统，该系统具有优化的照明和增加的横向分辨率约为3µm。将高速相位随机化与数值平均相结合，可显著提高信噪比。这种增强的有效性是通过散射物体的体积成像来证明的，它可以在细胞水平上对人体视网膜进行体内成像。此外，该实验得到了计算像差校正技术的支持，以实现人类视网膜的高分辨率体内成像。在平均过程中通过对比度增强实现视网膜锥体嵌合体和神经节细胞体的可视化。

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Imaging of retinal ganglion cells and photoreceptors using Spatio-Temporal Optical Coherence Tomography (STOC-T) without hardware-based adaptive optics

We demonstrate an experimental Spatio-Temporal Optical Coherence Tomography (STOC-T) system featuring optimized illumination and an increased lateral resolution of approximately 3 µm. The integration of high-speed phase randomization with a numerical averaging process facilitates a noticeable improvement in the signal-to-noise ratio. The effectiveness of this enhancement is demonstrated through volumetric imaging of a scattering object, and it enables in vivo imaging of the human retina at the cellular level. Additionally, the experiment is supported by computational aberration-correction techniques to achieve high-resolution in vivo imaging of the human retina. The visualization of retinal cone mosaics, and ganglion cell somas was achieved through contrast enhancement during the averaging process.

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来源期刊

Biocybernetics and Biomedical Engineering ENGINEERING, BIOMEDICAL-

CiteScore

16.50

自引率

6.20%

发文量

审稿时长

38 days

期刊介绍： Biocybernetics and Biomedical Engineering is a quarterly journal, founded in 1981, devoted to publishing the results of original, innovative and creative research investigations in the field of Biocybernetics and biomedical engineering, which bridges mathematical, physical, chemical and engineering methods and technology to analyse physiological processes in living organisms as well as to develop methods, devices and systems used in biology and medicine, mainly in medical diagnosis, monitoring systems and therapy. The Journal''s mission is to advance scientific discovery into new or improved standards of care, and promotion a wide-ranging exchange between science and its application to humans.