High-Throughput Cardiomyocytes Biosensing: From Single-Parameter Detection to Integrated Mechano-Electrophysiological Platforms

Developing the in vitro cardiac sensing platform is promising to study cardiac physiology, disease mechanisms, drug development, and personalized medicine. Changes in the electrophysiological activity and contractile strength of cardiomyocytes are particularly important for generating accurate in vitro cardiac platforms. Existing platforms generally have in situ sensing capabilities to capture changes in the electrophysiological or mechanical behavior of cardiomyocytes. However, sensing platforms still face challenges in high-throughput detection and simultaneous mechano-electrophysiological detection. This review covers the latest progress and shortcomings from high-throughput single physiological parameter detection platforms to mechano-electrophysiological simultaneous detection sensing platforms. Finally, we discuss the future prospects of mechano-electrophysiological sensing platforms in cardiomyocyte detection in order to achieve a more accurate and efficient in vitro cardiac sensing platform to facilitate heart disease research and drug screening.