Bo Li, Zi-Han Jia, Bing-Yan Li, Ting Hou, Fan-Xiang Meng, Xue-Yao Pang, Ya-Mu Xia* and Wei-Wei Gao*,
{"title":"NIR-II/pH双响应CuHDB@CaP纳米球作为高效对抗细菌感染的自级联催化平台","authors":"Bo Li, Zi-Han Jia, Bing-Yan Li, Ting Hou, Fan-Xiang Meng, Xue-Yao Pang, Ya-Mu Xia* and Wei-Wei Gao*, ","doi":"10.1021/acsanm.4c0622510.1021/acsanm.4c06225","DOIUrl":null,"url":null,"abstract":"<p >The misuse of antibiotics has led to a dramatic increase in bacterial resistance, creating an urgent need for efficient antimicrobial agents with multiple mechanisms of action. In this study, Cu was modified with berberine derivatives, and a core–shell structure was successfully developed, resulting in a light/pH-responsive CuHDB@CaP nanotherapeutic platform. It achieves controlled release of CuHDB nanozymes, with CaP, which is highly stable in neutral or alkaline environments, preventing CuHDB leakage and effectively “switching off” the activity of nanozymes. Bacterial metabolism, which produces lactic and keto acids, lowers the pH of the wound microenvironment. Additionally, exposure to 1064 nm near-infrared (NIR) light causes the CaP shell structure to collapse, releasing CuHDB nanozymes and “switching on” their enzyme-like activity. This enables the CuHDB@CaP nanotherapeutic platform to be both safe and highly efficient. CuHDB exhibits not only a strong photothermal effect but also enhanced glutathione oxidase (GSHOx)-like and peroxidase (POD)-like enzyme activities. The GSHOx-like activity contributes to the generation of H<sub>2</sub>O<sub>2</sub>, and its excellent H<sub>2</sub>O<sub>2</sub> affinity allows it to efficiently catalyze the conversion of H<sub>2</sub>O<sub>2</sub> to a sufficient amount of •OH through POD-like activity. This self-cascading catalytic platform does not require the introduction of additional H<sub>2</sub>O<sub>2</sub> and also promotes the consumption of GSH in the wound infection microenvironment. CuHDB@CaP can kill up to 99.0% of MRSA and Amp<sup>r</sup><i>E. coli</i>, effectively inhibit biofilm formation without the emergence of drug resistance, and exhibit negligible cytotoxicity and hemolysis. Treatment experiments in mouse wound infection models showed that CuHDB@CaP combined with NIR could effectively treat wound infections and accelerate wound healing with minimal toxicity to normal tissue cells, demonstrating its potential for the clinical treatment of skin infections.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 1","pages":"793–804 793–804"},"PeriodicalIF":5.5000,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"NIR-II/pH Dual Responsive CuHDB@CaP Nanospheres as a Self-Cascading Catalytic Platform for Highly Efficient Combating Bacterial Infections\",\"authors\":\"Bo Li, Zi-Han Jia, Bing-Yan Li, Ting Hou, Fan-Xiang Meng, Xue-Yao Pang, Ya-Mu Xia* and Wei-Wei Gao*, \",\"doi\":\"10.1021/acsanm.4c0622510.1021/acsanm.4c06225\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >The misuse of antibiotics has led to a dramatic increase in bacterial resistance, creating an urgent need for efficient antimicrobial agents with multiple mechanisms of action. In this study, Cu was modified with berberine derivatives, and a core–shell structure was successfully developed, resulting in a light/pH-responsive CuHDB@CaP nanotherapeutic platform. It achieves controlled release of CuHDB nanozymes, with CaP, which is highly stable in neutral or alkaline environments, preventing CuHDB leakage and effectively “switching off” the activity of nanozymes. Bacterial metabolism, which produces lactic and keto acids, lowers the pH of the wound microenvironment. Additionally, exposure to 1064 nm near-infrared (NIR) light causes the CaP shell structure to collapse, releasing CuHDB nanozymes and “switching on” their enzyme-like activity. This enables the CuHDB@CaP nanotherapeutic platform to be both safe and highly efficient. CuHDB exhibits not only a strong photothermal effect but also enhanced glutathione oxidase (GSHOx)-like and peroxidase (POD)-like enzyme activities. The GSHOx-like activity contributes to the generation of H<sub>2</sub>O<sub>2</sub>, and its excellent H<sub>2</sub>O<sub>2</sub> affinity allows it to efficiently catalyze the conversion of H<sub>2</sub>O<sub>2</sub> to a sufficient amount of •OH through POD-like activity. This self-cascading catalytic platform does not require the introduction of additional H<sub>2</sub>O<sub>2</sub> and also promotes the consumption of GSH in the wound infection microenvironment. CuHDB@CaP can kill up to 99.0% of MRSA and Amp<sup>r</sup><i>E. coli</i>, effectively inhibit biofilm formation without the emergence of drug resistance, and exhibit negligible cytotoxicity and hemolysis. Treatment experiments in mouse wound infection models showed that CuHDB@CaP combined with NIR could effectively treat wound infections and accelerate wound healing with minimal toxicity to normal tissue cells, demonstrating its potential for the clinical treatment of skin infections.</p>\",\"PeriodicalId\":6,\"journal\":{\"name\":\"ACS Applied Nano Materials\",\"volume\":\"8 1\",\"pages\":\"793–804 793–804\"},\"PeriodicalIF\":5.5000,\"publicationDate\":\"2024-12-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Nano Materials\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acsanm.4c06225\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Nano Materials","FirstCategoryId":"88","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsanm.4c06225","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
NIR-II/pH Dual Responsive CuHDB@CaP Nanospheres as a Self-Cascading Catalytic Platform for Highly Efficient Combating Bacterial Infections
The misuse of antibiotics has led to a dramatic increase in bacterial resistance, creating an urgent need for efficient antimicrobial agents with multiple mechanisms of action. In this study, Cu was modified with berberine derivatives, and a core–shell structure was successfully developed, resulting in a light/pH-responsive CuHDB@CaP nanotherapeutic platform. It achieves controlled release of CuHDB nanozymes, with CaP, which is highly stable in neutral or alkaline environments, preventing CuHDB leakage and effectively “switching off” the activity of nanozymes. Bacterial metabolism, which produces lactic and keto acids, lowers the pH of the wound microenvironment. Additionally, exposure to 1064 nm near-infrared (NIR) light causes the CaP shell structure to collapse, releasing CuHDB nanozymes and “switching on” their enzyme-like activity. This enables the CuHDB@CaP nanotherapeutic platform to be both safe and highly efficient. CuHDB exhibits not only a strong photothermal effect but also enhanced glutathione oxidase (GSHOx)-like and peroxidase (POD)-like enzyme activities. The GSHOx-like activity contributes to the generation of H2O2, and its excellent H2O2 affinity allows it to efficiently catalyze the conversion of H2O2 to a sufficient amount of •OH through POD-like activity. This self-cascading catalytic platform does not require the introduction of additional H2O2 and also promotes the consumption of GSH in the wound infection microenvironment. CuHDB@CaP can kill up to 99.0% of MRSA and AmprE. coli, effectively inhibit biofilm formation without the emergence of drug resistance, and exhibit negligible cytotoxicity and hemolysis. Treatment experiments in mouse wound infection models showed that CuHDB@CaP combined with NIR could effectively treat wound infections and accelerate wound healing with minimal toxicity to normal tissue cells, demonstrating its potential for the clinical treatment of skin infections.
期刊介绍:
ACS Applied Nano Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics and biology relevant to applications of nanomaterials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important applications of nanomaterials.