NIR-II/pH双响应CuHDB@CaP纳米球作为高效对抗细菌感染的自级联催化平台

IF 5.5 2区 材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY
Bo Li, Zi-Han Jia, Bing-Yan Li, Ting Hou, Fan-Xiang Meng, Xue-Yao Pang, Ya-Mu Xia* and Wei-Wei Gao*, 
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引用次数: 0

摘要

抗生素的滥用导致细菌耐药性急剧增加,迫切需要具有多种作用机制的高效抗菌药物。在本研究中,用小檗碱衍生物修饰Cu,并成功开发了核壳结构,从而获得了光/ ph响应CuHDB@CaP纳米治疗平台。它实现了CuHDB纳米酶的控制释放,具有CaP,在中性或碱性环境中高度稳定,防止CuHDB泄漏,有效地“关闭”纳米酶的活性。细菌代谢产生乳酸和酮酸,降低了伤口微环境的pH值。此外,暴露在1064nm的近红外(NIR)光下会导致CaP外壳结构崩溃,释放CuHDB纳米酶并“开启”它们的酶样活性。这使得CuHDB@CaP纳米治疗平台既安全又高效。CuHDB不仅具有较强的光热效应,还能增强谷胱甘肽氧化酶(GSHOx)样酶和过氧化物酶(POD)样酶的活性。gshox样活性有助于H2O2的生成,其优异的H2O2亲和力使其能够通过pod样活性有效地催化H2O2转化为足够数量的•OH。这种自级联催化平台不需要引入额外的H2O2,也促进了伤口感染微环境中谷胱甘肽的消耗。CuHDB@CaP可以杀死高达99.0%的MRSA和AmprE。大肠杆菌,有效地抑制生物膜的形成而不出现耐药性,并表现出微不足道的细胞毒性和溶血作用。小鼠伤口感染模型的治疗实验表明,CuHDB@CaP联合NIR可有效治疗伤口感染,加速伤口愈合,且对正常组织细胞的毒性最小,显示了其临床治疗皮肤感染的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

NIR-II/pH Dual Responsive CuHDB@CaP Nanospheres as a Self-Cascading Catalytic Platform for Highly Efficient Combating Bacterial Infections

NIR-II/pH Dual Responsive CuHDB@CaP Nanospheres as a Self-Cascading Catalytic Platform for Highly Efficient Combating Bacterial Infections

The misuse of antibiotics has led to a dramatic increase in bacterial resistance, creating an urgent need for efficient antimicrobial agents with multiple mechanisms of action. In this study, Cu was modified with berberine derivatives, and a core–shell structure was successfully developed, resulting in a light/pH-responsive CuHDB@CaP nanotherapeutic platform. It achieves controlled release of CuHDB nanozymes, with CaP, which is highly stable in neutral or alkaline environments, preventing CuHDB leakage and effectively “switching off” the activity of nanozymes. Bacterial metabolism, which produces lactic and keto acids, lowers the pH of the wound microenvironment. Additionally, exposure to 1064 nm near-infrared (NIR) light causes the CaP shell structure to collapse, releasing CuHDB nanozymes and “switching on” their enzyme-like activity. This enables the CuHDB@CaP nanotherapeutic platform to be both safe and highly efficient. CuHDB exhibits not only a strong photothermal effect but also enhanced glutathione oxidase (GSHOx)-like and peroxidase (POD)-like enzyme activities. The GSHOx-like activity contributes to the generation of H2O2, and its excellent H2O2 affinity allows it to efficiently catalyze the conversion of H2O2 to a sufficient amount of •OH through POD-like activity. This self-cascading catalytic platform does not require the introduction of additional H2O2 and also promotes the consumption of GSH in the wound infection microenvironment. CuHDB@CaP can kill up to 99.0% of MRSA and AmprE. coli, effectively inhibit biofilm formation without the emergence of drug resistance, and exhibit negligible cytotoxicity and hemolysis. Treatment experiments in mouse wound infection models showed that CuHDB@CaP combined with NIR could effectively treat wound infections and accelerate wound healing with minimal toxicity to normal tissue cells, demonstrating its potential for the clinical treatment of skin infections.

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来源期刊
CiteScore
8.30
自引率
3.40%
发文量
1601
期刊介绍: ACS Applied Nano Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics and biology relevant to applications of nanomaterials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important applications of nanomaterials.
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