膜力学性能的皮流控电渗透测量

IF 12.1 2区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Small Pub Date : 2025-02-03 DOI:10.1002/smll.202410095
Xiao-Yuan Wang, Ze-Rui Zhou, Li-Juan Gong, Man-Sha Wu, Shi-Yi Zhang, Jian Lv, Bin-Bin Chen, Da-Wei Li, Ruo-Can Qian
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引用次数: 0

摘要

细胞通过质膜与内外环境联系,细胞膜的力学特性支配着许多生物事件。膜检测技术,如光学或磁性镊子,已经通过膜锚定修饰揭示了机械强度,但开发无标签方法来减少外源干扰的影响仍然具有挑战性。本文介绍了皮流控电渗透测量(peofluidic electro-osmosis measurement,简称PEOM)技术,该技术通过使用玻璃纳米管而无需标记,可以直接有效地检测细胞膜的机械性能。通过在纳米吸管尖端产生皮升的电渗透流体,从电流迹中观察到周期性的细胞膜振动模式,这些振动模式携带着膜力学特性的信息,以指示其生物状态。基于频域特征峰,建立了描述膜振动模式的理论框架,该框架包含两个理想的弹簧振子模型,分别对应于细胞膜的拉伸振动和弯曲振动。值得注意的是,PEOM策略代表了一种无标签的方法,可以从二维角度揭示活细胞膜的力学特性,这与其他方法完全不同。此外,在不同的生物过程中,包括细胞骨架改变、膜张力变化和机械极化等膜力学特性变化的无标记观察中,证明了PEOM的令人兴奋的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Picofluidic Electro-Osmosis Measurement of Cell Membrane Mechanical Properties

Picofluidic Electro-Osmosis Measurement of Cell Membrane Mechanical Properties

Picofluidic Electro-Osmosis Measurement of Cell Membrane Mechanical Properties

Cells connect with their internal and external environments through plasma membranes, and the mechanical properties of cell membranes govern numerous biological events. Membrane detection techniques such as optical or magnetic tweezers have revealed mechanical strength by membrane-anchored modifications, but it remains challenging to develop label-free methods to reduce the influence of exogenous interference. Here picofluidic electro-osmosis measurement (PEOM), which enables direct and efficient sensing of cell membrane mechanical properties by using a glass nanopipette without labeling, is presented. By generating a picoliter electroosmotic fluid at the nanopipette tip, periodic cell membrane vibration modes are observed from current traces, which carry information on membrane mechanical properties to indicate its biological state. Based on characteristic peaks in the frequency domain, a theoretical framework to describe the vibration modes, which contains two ideal spring vibrator models corresponding to stretching and bending vibrations of cell membrane respectively, is developed. Notably, the PEOM strategy represents a label-free approach to reveal the mechanical properties of living cell membranes from two dimensions, which is completely different from other methods. Additionally, the exciting potential of PEOM is demonstrated for label-free observation of membrane mechanical property changes during different bioprocesses, including cytoskeletal alteration, membrane tension change, and mechanical polarization.

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来源期刊
Small
Small 工程技术-材料科学:综合
CiteScore
17.70
自引率
3.80%
发文量
1830
审稿时长
2.1 months
期刊介绍: Small serves as an exceptional platform for both experimental and theoretical studies in fundamental and applied interdisciplinary research at the nano- and microscale. The journal offers a compelling mix of peer-reviewed Research Articles, Reviews, Perspectives, and Comments. With a remarkable 2022 Journal Impact Factor of 13.3 (Journal Citation Reports from Clarivate Analytics, 2023), Small remains among the top multidisciplinary journals, covering a wide range of topics at the interface of materials science, chemistry, physics, engineering, medicine, and biology. Small's readership includes biochemists, biologists, biomedical scientists, chemists, engineers, information technologists, materials scientists, physicists, and theoreticians alike.
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