骶神经根磁刺激对神经源性膀胱大鼠膀胱尿动力学及M3受体表达的影响。

IF 2 Q3 NEUROSCIENCES

IBRO Neuroscience Reports Pub Date : 2025-01-14 DOI:10.1016/j.ibneur.2025.01.010

Junhua Li, Chenhao Tang, Longfei Yang, Chen Song, Yanbin Wang

{"title":"骶神经根磁刺激对神经源性膀胱大鼠膀胱尿动力学及M3受体表达的影响。","authors":"Junhua Li, Chenhao Tang, Longfei Yang, Chen Song, Yanbin Wang","doi":"10.1016/j.ibneur.2025.01.010","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the effect of sacral nerve root magnetic stimulation(SMS) on bladder urodynamics and M3 receptor expression in rats with neurogenic bladder.</div></div><div><h3>Methods</h3><div>30 adult female SD rats were randomly divided into Normal Group, Model Group, and Magnetic Stimulation Group, with 10 rats in each group. The Model Group and Magnetic Stimulation Group used the spinal cord transtion method to establish the neurogenic bladder animal model. After successful modeling, the Magnetic Stimulation Group received magnetic stimulation treatment once per day for 8 consutive weeks. After 8 weeks, bladder urodynamics were measured under anesthesia, rats were sacrificed, and bladder detrusor muscle tissue was collected for histological and ultrastructural observation, and M3 receptor expression levels were measured.</div></div><div><h3>Results</h3><div>The maximum bladder capacity and bladder compliance in the Magnetic Stimulation Group were higher than those in the Model Group (all <em>P</em> < 0. 05), and the leak point pressure was lower than that in the Model Group (P < 0. 05); there were no significant differences between the Magnetic Stimulation Group and the Normal Group in these three parameters (all <em>P</em> > 0. 05). H&E staining of bladder detrusor muscle tissue in the Magnetic Stimulation Group revealed minimal neutrophil infiltration. Moreover, the morphology and arrangement of the mucosal epithelial cells were closer to those observed in the Normal Group when compared with the Model Group. Under transmission electron microscopy, detrusor muscle cells had a smooth surface, slightly widened intercellular spaces, relatively uniform arrangement, and relatively intact mitochondrial structure. The expression level of M3 receptors in the bladder detrusor muscle tissue of the Magnetic Stimulation Group was significantly higher than that in the Normal Group and the Model Group (all <em>P</em> < 0. 05); there was no significant difference between the Model Group and the Normal Group (<em>P</em> > 0. 05).</div></div><div><h3>Conclusion</h3><div>Sacral nerve root magnetic stimulation has a certain effect on improving bladder function in rats with neurogenic bladder, which may be related to the increased expression level of M3 receptors in the bladder detrusor muscle tissue.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"18 ","pages":"Pages 158-162"},"PeriodicalIF":2.0000,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786741/pdf/","citationCount":"0","resultStr":"{\"title\":\"The effect of sacral nerve root magnetic stimulation on bladder urodynamics and M3 receptor expression in rats with neurogenic bladder\",\"authors\":\"Junhua Li, Chenhao Tang, Longfei Yang, Chen Song, Yanbin Wang\",\"doi\":\"10.1016/j.ibneur.2025.01.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>To investigate the effect of sacral nerve root magnetic stimulation(SMS) on bladder urodynamics and M3 receptor expression in rats with neurogenic bladder.</div></div><div><h3>Methods</h3><div>30 adult female SD rats were randomly divided into Normal Group, Model Group, and Magnetic Stimulation Group, with 10 rats in each group. The Model Group and Magnetic Stimulation Group used the spinal cord transtion method to establish the neurogenic bladder animal model. After successful modeling, the Magnetic Stimulation Group received magnetic stimulation treatment once per day for 8 consutive weeks. After 8 weeks, bladder urodynamics were measured under anesthesia, rats were sacrificed, and bladder detrusor muscle tissue was collected for histological and ultrastructural observation, and M3 receptor expression levels were measured.</div></div><div><h3>Results</h3><div>The maximum bladder capacity and bladder compliance in the Magnetic Stimulation Group were higher than those in the Model Group (all <em>P</em> < 0. 05), and the leak point pressure was lower than that in the Model Group (P < 0. 05); there were no significant differences between the Magnetic Stimulation Group and the Normal Group in these three parameters (all <em>P</em> > 0. 05). H&E staining of bladder detrusor muscle tissue in the Magnetic Stimulation Group revealed minimal neutrophil infiltration. Moreover, the morphology and arrangement of the mucosal epithelial cells were closer to those observed in the Normal Group when compared with the Model Group. Under transmission electron microscopy, detrusor muscle cells had a smooth surface, slightly widened intercellular spaces, relatively uniform arrangement, and relatively intact mitochondrial structure. The expression level of M3 receptors in the bladder detrusor muscle tissue of the Magnetic Stimulation Group was significantly higher than that in the Normal Group and the Model Group (all <em>P</em> < 0. 05); there was no significant difference between the Model Group and the Normal Group (<em>P</em> > 0. 05).</div></div><div><h3>Conclusion</h3><div>Sacral nerve root magnetic stimulation has a certain effect on improving bladder function in rats with neurogenic bladder, which may be related to the increased expression level of M3 receptors in the bladder detrusor muscle tissue.</div></div>\",\"PeriodicalId\":13195,\"journal\":{\"name\":\"IBRO Neuroscience Reports\",\"volume\":\"18 \",\"pages\":\"Pages 158-162\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-01-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786741/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IBRO Neuroscience Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667242125000107\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IBRO Neuroscience Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667242125000107","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NEUROSCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

目的：探讨骶神经根磁刺激对神经源性膀胱大鼠膀胱尿动力学及M3受体表达的影响。方法：将30只成年雌性SD大鼠随机分为正常组、模型组和磁刺激组，每组10只。模型组和磁刺激组采用脊髓转移法建立神经源性膀胱动物模型。造模成功后，磁刺激组给予磁刺激治疗，每天1次，连续8周。8周后，麻醉下测定膀胱尿动力学，处死大鼠，采集膀胱逼尿肌组织进行组织学和超微结构观察，测定M3受体表达水平。结果：磁刺激组大鼠膀胱最大容量、膀胱顺应性均高于模型组（P均 P > 0）。05)。磁刺激组膀胱逼尿肌组织H&E染色显示微量中性粒细胞浸润。与模型组比较，大鼠粘膜上皮细胞的形态和排列更接近正常组。透射电镜下，逼尿肌细胞表面光滑，细胞间隙略宽，排列相对均匀，线粒体结构相对完整。磁刺激组膀胱逼尿肌组织中M3受体表达水平显著高于正常组和模型组（均P P > 0）。05)。结论：骶神经根磁刺激对神经源性膀胱大鼠的膀胱功能有一定的改善作用，可能与膀胱逼尿肌组织中M3受体表达水平升高有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

The effect of sacral nerve root magnetic stimulation on bladder urodynamics and M3 receptor expression in rats with neurogenic bladder

Objective

To investigate the effect of sacral nerve root magnetic stimulation(SMS) on bladder urodynamics and M3 receptor expression in rats with neurogenic bladder.

Methods

30 adult female SD rats were randomly divided into Normal Group, Model Group, and Magnetic Stimulation Group, with 10 rats in each group. The Model Group and Magnetic Stimulation Group used the spinal cord transtion method to establish the neurogenic bladder animal model. After successful modeling, the Magnetic Stimulation Group received magnetic stimulation treatment once per day for 8 consutive weeks. After 8 weeks, bladder urodynamics were measured under anesthesia, rats were sacrificed, and bladder detrusor muscle tissue was collected for histological and ultrastructural observation, and M3 receptor expression levels were measured.

Results

The maximum bladder capacity and bladder compliance in the Magnetic Stimulation Group were higher than those in the Model Group (all P < 0. 05), and the leak point pressure was lower than that in the Model Group (P < 0. 05); there were no significant differences between the Magnetic Stimulation Group and the Normal Group in these three parameters (all P > 0. 05). H&E staining of bladder detrusor muscle tissue in the Magnetic Stimulation Group revealed minimal neutrophil infiltration. Moreover, the morphology and arrangement of the mucosal epithelial cells were closer to those observed in the Normal Group when compared with the Model Group. Under transmission electron microscopy, detrusor muscle cells had a smooth surface, slightly widened intercellular spaces, relatively uniform arrangement, and relatively intact mitochondrial structure. The expression level of M3 receptors in the bladder detrusor muscle tissue of the Magnetic Stimulation Group was significantly higher than that in the Normal Group and the Model Group (all P < 0. 05); there was no significant difference between the Model Group and the Normal Group (P > 0. 05).

Conclusion

Sacral nerve root magnetic stimulation has a certain effect on improving bladder function in rats with neurogenic bladder, which may be related to the increased expression level of M3 receptors in the bladder detrusor muscle tissue.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊