布鲁氏菌病多价DNA疫苗的研制及疗效评价

Q3 Agricultural and Biological Sciences

Doklady Biological Sciences Pub Date : 2025-02-01 Epub Date: 2025-01-31 DOI:10.1134/S0012496624600544

Y M Khodarovich, Maha A Elshazly, W S Shell, S A Nassif, D A Devrishov, S A Magomedova, S N Marzanova, S M Deyev

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引用次数: 0

摘要

布鲁氏菌减毒活菌株目前用于布鲁氏菌病疫苗，对动物造成严重副作用，几乎不适合用于人类疫苗接种。为了开发更安全的布鲁氏菌病疫苗，构建了在哺乳动物细胞中表达6种布鲁氏菌蛋白的质粒DNA。线性聚乙烯亚胺用于提高DNA进入细胞的效率。介绍了dna -聚乙烯亚胺络合物形成条件的优化数据。单次肌内或皮下注射低剂量（20 μg/小鼠）的聚乙烯亚胺质粒DNA复合物，对随后的布鲁氏菌试验菌株的攻击显示出可靠的保护作用。

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Development and Efficacy Evaluation of Multivalent DNA Vaccine against Brucellosis.

Attenuated live Brucella strains are currently used in vaccines against brucellosis, causing serious side effects in animals and being hardly suitable for human vaccination. To develop a safer vaccine against brucellosis, plasmid DNA was constructed to express six Brucella proteins in mammalian cells. Linear polyethylenimine was used to increase the efficiency of DNA delivery into cells. Data on optimization of the conditions for the formation of DNA-polyethylenimine complexes were described. A single intramuscular or subcutaneous injection of plasmid DNA complexes with polyethylenimine at a low dose (20 μg/mouse) was shown to exert a reliable protective effect against subsequent challenge with a test Brucella strain.

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来源期刊

Doklady Biological Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Doklady Biological Sciences is a journal that publishes new research in biological sciences of great significance. Initially the journal was a forum of the Russian Academy of Science and published only best contributions from Russia in the form of short articles. Now the journal welcomes submissions from any country in the English or Russian language. Every manuscript must be recommended by Russian or foreign members of the Russian Academy of Sciences.