来自商业肉鸡养殖场环境的粪肠球菌CRISPR-Cas系统的特征及其与抗菌素耐药性的关系

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
M B Kim, Y J Lee
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引用次数: 0

摘要

1. 聚类规则间隔短回文重复(CRISPR)相关蛋白(Cas)系统因其在控制可移动遗传元件(包括抗菌素耐药性(AMR)基因)传播方面的潜在应用而受到重视。本研究调查了商业肉鸡养殖场粪肠杆菌中CRISPR-Cas系统的特征,并评估了这些系统对AMR.2的影响。所有粪肠杆菌分离株均含有CRISPR2,分别在84株(56.4%)和144株(96.6%)分离株中检测到CRISPR1-Cas和CRISPR3-Cas。在27个(96.4%)农场中分别鉴定出了CRISPR2和CRISPR3-Cas的组合,以及CRISPR1-Cas、CRISPR2和CRISPR3-Cas的组合。表型AMR的CRISPR-Cas系统:CRISPR1-Cas和CRISPR3-Cas之间存在显著差异。与含有crispr - cas的菌株相比,不含crispr - cas的菌株对大多数抗菌素具有更高的耐药性,并且具有更高的多药耐药(MDR)患病率。然而,对大多数抗菌素的耐药率和耐多药的患病率并没有显着差异,这取决于是否存在crispr - cas4。没有CRISPR1-Cas的粪肠球菌分离株比具有CRISPR1-Cas的分离株具有更高的AMR基因水平,除了tel。然而,携带CRISPR3-Cas的粪肠球菌分离株中,tetL基因的流行率明显较低,而fexA和poxtA基因的流行率明显较高。在rep家族分布中,以rep9家族居多,其次为rep1、rep7、rep2和rep8家族。只有未携带CRISPR1-Cas的粪肠球菌中rep7家族的患病率(15.4%)显著高于携带CRISPR1-Cas的粪肠球菌(0%)。该研究首次报道了从商业肉鸡养殖场环境中分离的粪肠杆菌中CRISPR-Cas系统的特征,结果支持了抗菌策略的发展需要了解CRISPR1-Cas和CRISPR3-Cas之间的独特能力及其潜在耐药机制的假设。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterisation of the CRISPR-Cas systems in Enterococcus faecalis from commercial broiler farm environments and its association with antimicrobial resistance.

1. Clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins (Cas) systems have been highlighted for their potential applications in controlling the spread of mobile genetic elements, including antimicrobial resistance (AMR) genes. This study investigated the characteristics of CRISPR-Cas systems in E. faecalis from commercial broiler farms and assessed the impact of these systems on AMR.2. All E. faecalis isolates contained CRISPR2, and CRISPR1-Cas and CRISPR3-Cas were identified in 84 (56.4%) and 144 (96.6%) isolates. A combination of CRISPR2 and CRISPR3-Cas and a combination of CRISPR1-Cas, CRISPR2 and CRISPR3-Cas were each identified in 27 (96.4%) farms.3. There were significant differences between CRISPR-Cas systems for phenotypic AMR: CRISPR1-Cas and CRISPR3-Cas. The E. faecalis isolates without CRISPR1-Cas showed higher resistance to most antimicrobials and had a higher prevalence of multidrug resistance (MDR) than those with CRISPR1-Cas. However, the resistance rate against most antimicrobials and the prevalence of MDR did not differ significantly depending on the presence or absence of CRISPR3-Cas.4. The E. faecalis isolates without CRISPR1-Cas harboured higher levels of all AMR genes, except for tetL, than those with CRISPR1-Cas. However, the E. faecalis isolates with CRISPR3-Cas showed a significant lower prevalence of tetL gene and a significantly higher prevalence of fexA and poxtA genes.5. In the distribution of rep families, the rep9 family was predominant, followed by rep1, rep7, rep2 and rep8 families. Only prevalence of the rep7 family was significantly higher in the E. faecalis isolates without CRISPR1-Cas (15.4%) than in those with CRISPR1-Cas (0%).6. This study is the first report on the characteristics of CRISPR-Cas systems in E. faecalis isolated from commercial broiler farm environments, and the results supported the hypothesis that the development of antimicrobial strategies requires an understanding of the distinctive capabilities between CRISPR1-Cas and CRISPR3-Cas and their underlying resistance mechanisms.

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来源期刊
British Poultry Science
British Poultry Science 农林科学-奶制品与动物科学
CiteScore
3.90
自引率
5.00%
发文量
88
审稿时长
4.5 months
期刊介绍: From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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