腹泻型肠易激综合征患者舌苔微生物群的改变:一项横断面研究。

IF 2.2 4区 医学 Q4 IMMUNOLOGY
Apmis Pub Date : 2025-02-03 DOI:10.1111/apm.70001
Yitong Li, Yuhe Mai, Yao Jiao, Yali Yuan, Yingdi Qu, Ye Zhang, Muyuan Wang, Wenji Zhang, Xinyu Lu, Zhengdao Lin, Chengtao Liang, Junxiang Li, Tangyou Mao, Chune Xie
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引用次数: 0

摘要

肠道菌群在IBS-D的发生和发展中起着至关重要的作用,然而IBS-D相关的舌苔菌群失调尚未明确定义。为了解决这个问题,我们使用16S rRNA高通量测序分析了23名IBS-D患者和12名健康对照者的舌苔微生物组的结构和组成。16S rRNA测序结果显示,与健康对照组相比,IBS-D患者舌苔微生物组总体观察到的OTUs显著降低。α多样性分析显示,IBS-D患者的舌苔微生物群落多样性和丰富度显著降低,PCoA显示IBS-D患者与健康对照组之间存在明显的聚类。属水平上的微生物比较显示,IBS-D患者的细络菌(Veillonella)、普雷沃氏菌(Prevotella)的丰度高于健康对照组,而链球菌(Streptococcus)、嗜血杆菌(Haemophilus)、肉芽菌(Granulicatella)和罗氏菌(Rothia)的丰度明显低于健康对照组。功能分析结果显示,IBS-D患者与健康对照者在88条功能性代谢途径中存在显著差异,其中包括脂肪酸生物合成。这些发现确定了IBS-D患者舌苔微生物群的结构、组成和功能,并有望在IBS-D治疗期间成为潜在的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alterations in the Tongue Coating Microbiome in Patients With Diarrhea-Predominant Irritable Bowel Syndrome: A Cross-Sectional Study

The gut microbiota plays a critical role in the occurrence and development of IBS-D, however, IBS-D-associated tongue coating microbiome dysbiosis has not yet been clearly defined. To address this, we analyzed the structure and composition of the tongue coating microbiome in 23 IBS-D patients and 12 healthy controls using 16S rRNA high-throughput sequencing analysis. The 16S rRNA sequencing results revealed that the overall observed OTUs of tongue coating microbiome in IBS-D patients exhibited a significant decrease compared with the healthy controls. Alpha diversity analysis showed that the diversity and community richness were significantly reduced in IBS-D patients, and PCoA revealed a distinct clustering of tongue coating microbiome between the IBS-D patients and healthy controls. Microbial comparisons at the genus level showed that the abundance of Veillonella, Prevotella in IBS-D patients was higher than those in healthy controls, while Streptococcus, Haemophilus, Granulicatella, and Rothia were significantly reduced compared with the healthy volunteers. Functional analysis results showed significant differences in 88 functional metabolic pathways between the IBS-D patients and the healthy controls, including fatty acid biosynthesis. These findings identified the structure, composition, functionality of tongue coating microbiome in IBS-D patients, and hold promise the potential for therapeutic targets during IBS-D management.

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来源期刊
Apmis
Apmis 医学-病理学
CiteScore
5.20
自引率
0.00%
发文量
91
审稿时长
2 months
期刊介绍: APMIS, formerly Acta Pathologica, Microbiologica et Immunologica Scandinavica, has been published since 1924 by the Scandinavian Societies for Medical Microbiology and Pathology as a non-profit-making scientific journal.
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