超声与血清学对实验感染绵羊囊性包虫病诊断及治疗后监测的比较分析。

IF 2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-02-01 DOI:10.1016/j.vetpar.2025.110406

Figen Celik , Sami Simsek , Songul Ceribasi , Muhammet Uslug , Mehmet Cengiz Han

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引用次数: 0

摘要

囊性棘球蚴病是全球最重要的人畜共患疾病之一，影响人类和牲畜。本研究的目的是比较超声（US）和血清学方法作为实验感染绵羊CE感染后和治疗后随访的早期诊断标志物的有效性。抽吸包虫囊液，并给狗口服20,000个可存活的原头节。50 d后，收集所有成虫。取卵对14只羔羊（11只实验羔羊，3只对照羔羊）进行实验感染。采集血样，每周行超声显像。制备了部分纯化的包虫囊液（PPHCF）和重组副肌球蛋白（EgPmy5）抗原。PPHCFA的敏感性（95 %）高于rEgPmy5(70 %)，但与其他寄生虫感染的交叉反应率分别为95 %和20 %。在感染后8个月首次行超声显像。实验感染第8个月开始阿苯达唑（7.5 mg/kg）治疗。6只羊每30天用阿苯达唑治疗，为期3个月。在治疗第8天和第10天死亡的两只羊中，观察到由于肝脏囊肿中液体减少和纤维化，囊肿膜张力消失。经过三个月的治疗，大部分肝脏囊肿的大小缩小到针头大小，与对照组相比，囊肿的总数减少了。治疗6个月后，肝脏出现无液体的针尖大小的纤维化囊肿。从实验感染的第7天起，一只PPHCFA羊和另一只rEgPmy5羊的血清检测呈阳性，并一直检测到死亡。综上所述，阿苯达唑早在治疗第1周就能诱导包虫病囊变形，PPHCF抗原可用于羊群体血清学监测。

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A comparative analysis of ultrasound and serology for the diagnosis and post-treatment monitoring of cystic echinococcosis in experimentally infected sheep

Cystic echinococcosis (CE) is one of the most significant zoonotic diseases globally, affecting both humans and livestocks. The aim of this study was to compare the efficacy of ultrasound (US) and serological methods as early diagnostic markers for both post-infection and post-treatment follow-up of CE in experimentally infected sheep. An hydatid cyst fluid was aspirated and 20,000 viable protoscoleces were administered orally to a dog. After 50 days, all adult parasites were collected. The eggs were extracted and used for the experimental infection of 14 lambs (11 experimental and 3 control). Blood samples were collected and US imaging were performed weekly. Partially purified hydatid cyst fluid (PPHCF) and recombinant paramyosin (EgPmy5) antigens were prepared. The sensitivity of PPHCFA (95 %) was observed to be higher than that of rEgPmy5 (70 %), yet the cross-reaction rates with other helminth infections were 95 % and 20 %, respectively. The cysts first time were imaged at the 8 month after infection by US. Albendazole (7.5 mg/kg) treatment was initiated in the eighth month of the experimental infection. Six sheep were treated with albendazole every 30 days for a period of three months. In two sheep which died on the 8th and 10th day of the treatment, it was observed that the tension in the cyst membrane had disappeared due to a reduction in fluid and fibrosis in the cysts in the liver. Following a three-month treatment, the majority of cysts in the liver had reduced in size to approximately that of a pinhead, and the overall number of cysts had decreased in comparison with the control sheep. After a six-month treatment the presence of fibrotic, pinhead-sized cysts devoid of fluid was observed in the liver. Seropositivity was detected in a sheep with PPHCFA and another sheep with rEgPmy5 from the seventh day of experimental infection and remained detectable till death. In conclusion, albendazole can induce deformation in hydatid cysts as early as the first week of treatment and PPHCF antigen can be used for serological monitoring of the sheep population.

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.