揭示隐性鼻病毒感染在慢性鼻窦炎伴鼻息肉中的流行及影响。

IF 5.8 2区医学 Q1 ALLERGY

Annals of Allergy Asthma & Immunology Pub Date : 2025-04-01 DOI:10.1016/j.anai.2025.01.026

Xinyi Ma MBBS , Shu Guo MBBS , Fangying Liu MBBS , Changqing Li MBBS , Xueyun Shi MBBS , Weiyuan Liu MBBS , Lijie Qi MD , Ye Yuan MBBS , Xinyu Xie MBBS , Pin Wang PhD , Larry Borish MD , Xin Feng MD

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引用次数: 0

摘要

背景：慢性鼻窦炎伴鼻息肉（CRSwNP）涉及持续性鼻窦炎症，新出现的证据表明鼻病毒（RV）在其病理生理中的潜在作用。然而，RV是否存在于鼻组织并在感染症状消退后影响鼻黏膜尚不清楚。目的：探讨鼻组织隐匿性RV感染的流行情况及影响。方法：对47例对照组和101例无呼吸道感染的CRSwNP患者进行鼻组织RV负荷检测。参与者被分为rv阳性（+），rv阴性（-）和“灰色地带”组。采用定量PCR （qPCR）、Western Blot和免疫荧光法分析RV沉默感染对鼻组织免疫状态的影响。结果：隐匿性RV感染在对照组（34%）和CRSwNP（30.7%）组织中普遍存在，在鼻息肉中观察到更高的病毒载量。在对照组中，它与Ⅰ型和Ⅱ型干扰素、2型炎症、白细胞介素- 17a和白细胞介素-10的高表达有关。在CRSwNP患者中，沉默型RV感染与较低的I型IFN、IL-17A、2型炎症和IL-10相关，但与未感染RV的患者相比，II型IFN较高。RV（+）型鼻息肉组织嗜酸性粒细胞和中性粒细胞明显少于RV（-）型鼻息肉。结论：RV感染在鼻组织中普遍存在，在鼻息肉中检测到较高的病毒载量。在健康对照和CRSwNP患者中，这种沉默的鼻病毒感染与不同的免疫反应相关，涉及ifn、Th2细胞因子、IL-17A、IL-10以及嗜酸性粒细胞和中性粒细胞水平的差异调节。

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Unveiling the prevalence and impact of silent rhinovirus infection in chronic rhinosinusitis with nasal polyps

Background

Chronic rhinosinusitis with nasal polyps (CRSwNPs) involves persistent sinus inflammation, with emerging evidence suggesting a potential role of rhinovirus (RV) in its pathophysiology. However, whether RV exists in nasal tissues and affects the nasal mucosa after the resolution of infection symptoms remains unknown.

Objective

To investigate the prevalence and impact of silent RV infection in nasal tissues.

Methods

RV loads were detected in the nasal tissues of 47 controls and 101 patients with CRSwNP without respiratory infection. Participants were categorized into RV-positive (+), RV-negative (−), and the “gray zone” groups. Quantitative polymerase chain reaction, Western blotting, and immunofluorescence assays were used to analyze the impact of silent RV infection on the immune status of nasal tissues.

Results

Silent RV infection was prevalent in both control (34%) and CRSwNP (30.7%) tissues, with higher viral loads observed in the nasal polyps. In controls, it was associated with high expression of types 1 and 2 interferon (IFN), type 2 inflammation, interleukin (IL)-17A, and IL-10. In patients with CRSwNP, silent RV infection was associated with lower levels of type 1 IFN, IL-17A, type 2 inflammation, and IL-10 but higher levels of type 2 IFN compared with those without RV infection. Meanwhile, RV (+) nasal polyps exhibited fewer tissue eosinophils and neutrophils than RV (−) nasal polyps.

Conclusion

Silent RV infection was prevalent in the nasal tissues, with a higher viral load detected in the nasal polyps. This silent RV infection is associated with distinct immune responses in healthy controls and patients with CRSwNP, involving differential modulation of IFNs, T_H2 cytokines, IL-17A, IL-10, and eosinophil and neutrophil levels.

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来源期刊

Annals of Allergy Asthma & Immunology 医学-过敏

CiteScore

6.50

自引率

6.80%

发文量

437

审稿时长

33 days

期刊介绍： Annals of Allergy, Asthma & Immunology is a scholarly medical journal published monthly by the American College of Allergy, Asthma & Immunology. The purpose of Annals is to serve as an objective evidence-based forum for the allergy/immunology specialist to keep up to date on current clinical science (both research and practice-based) in the fields of allergy, asthma, and immunology. The emphasis of the journal will be to provide clinical and research information that is readily applicable to both the clinician and the researcher. Each issue of the Annals shall also provide opportunities to participate in accredited continuing medical education activities to enhance overall clinical proficiency.