两种来自年轻人和老年人外周血的人类诱导多能干细胞系的产生、表征和验证。

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-01-27 DOI:10.1016/j.scr.2025.103670

Dongli Yang , Jun Chen , Jerry H. Juratli , Andre Monteiro da Rocha , Allison Schley , Nadia R. Sutton

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引用次数: 0

摘要

衰老是发展与年龄有关的疾病的主要危险因素。然而，衰老如何影响人类诱导多能干细胞（hiPSC）重编程、年龄相关的重编程后表观遗传记忆、分化及其在心血管再生医学中的潜在适用性仍未得到充分探讨。我们从年轻和老年献血者的全血中获得人外周血单个核细胞（PBMCs），生成、表征并验证了两种hiPSC系。这两种hiPSC细胞系表达4种多能性标记，具有正常的核型和三代分化潜力，遗传上与亲本PBMCs匹配。这些细胞系对于再生医学和探索衰老和衰老相关疾病背后的表观遗传相关分子机制是无价的。

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Generation, characterization, and validation of two human induced pluripotent stem cell lines from the peripheral blood of young and older adults

Aging is a leading risk factor for the development of age-related diseases. However, how aging impacts human induced pluripotent stem cell (hiPSC) reprogramming, age-related epigenetic memory post-reprogramming, differentiation, and its potential applicability to cardiovascular regenerative medicine remains underexplored. We generated, characterized, and validated two hiPSC lines from human peripheral blood mononuclear cells (PBMCs) obtained from whole blood of young and older human donors. The two hiPSC lines expressed four pluripotency markers, have normal karyotypes and trilineage differentiation potential, and genetically match parental PBMCs. These lines are invaluable for regenerative medicine and exploring epigenetic-related molecular mechanisms that underlie aging and aging-related diseases.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.