含有blaNDM-1基因的耐碳青霉烯鲍曼不动杆菌ST600的流行病学和基因组特征，波兰首次报道。

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2025-01-30 DOI:10.1016/j.jgar.2025.01.014

Estera Jachowicz-Matczak , Tomasz Wołkowicz , Anna Kujawska , Anna Pałka , Mateusz Gajda , Barbara Żółtowska , Katarzyna Zacharczuk , Katarzyna Piekarska , Jakub Kasprzyk , Natalia Wieczorek , Rafał Gierczyński , Jadwiga Wójkowska-Mach

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引用次数: 0

摘要

背景：鲍曼不动杆菌（Ab）在重症监护病房（ICU）和机械通气患者中引起严重感染。根据欧洲疾病预防和控制中心2018年的数据，波兰icu中Ab的患病率高于其他欧洲国家，而在2021年，近40%的Ab对碳青霉烯类（CR-Ab）具有耐药性。我们的目的是通过全基因组测序（WGS）和表型检测来研究从波兰克拉科夫大学医院收集的CR-Ab株的流行病学和分子特征。方法：对2019年（442例）和2021年（235例）收集的667例医院获得性（HAI） CR-Ab感染的流行病学和耐药数据进行分析。进行细菌鉴定、药敏及碳青霉烯酶检测。WGS对2021年分离的125例CR-Ab进行了研究。结果：在WGS检测的125株CR-Ab中，绝大多数104株（82%）属于最常见的ST2，而20株（16%）属于很少报道的ST600。对碳青霉烯类耐药基因最多的是blaOXA-66（96.8%）和blaOXA-72(71.2%)，而ST600菌株特有的基因为blaOXA-23和blaNDM-1。blaNDM-1基因位于Tn125的IS30染色体上。结论：我们的研究揭示了在波兰首次报道的偶尔报道的携带blaNDM-1基因的ST600中存在CR-Ab。应实施常规基因组监测，以监测和减少循环耐药Ab克隆的传播。

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Epidemiological and genomic characterization of carbapenem-resistant Acinetobacter baumannii ST600 harbouring the blaNDM-1 gene, first report in Poland

Objectives

Acinetobacter baumannii (A. baumannii) causes severe infections among intensive care units (ICUs) and patients who are mechanically ventilated. According to the European Centre for Disease Prevention and Control data for 2018 the prevalence of A. baumannii in Polish ICUs was higher than in other European countries, whereas almost 40 % of A. baumannii were resistant to carbapenems (CR-Ab) in 2021. Our goal was to investigate the epidemiology and molecular characteristics of CR-Ab strains collected from the University Hospital in Krakow, Poland, by whole-genome sequencing (WGS) and phenotypic tests.

Methods

Epidemiological and drug-resistance data from a total of 667 hospital-acquired (HAI) CR-Ab infections collected in 2019 (442) and 2021 (235) were examined. Bacterial identification, antimicrobial susceptibility, and detection of carbapenemases were performed. A selected subset of 125 CR-Ab isolated in 2021 was investigated by WGS.

Results

Among 125 CR-Ab isolates examined by WGS, the vast majority 104 (82 %), belonged to the most prevalent ST2, and 20 (16 %) belonged to the rarely reported ST600. The most common genes conferring resistance to carbapenems were bla_OXA-66 (96.8 %), and bla_OXA-72 (71.2 %), whereas the bla_OXA-23 and bla_NDM-1 genes were specific to ST600 isolates. The bla_NDM-1 gene was chromosomally located in IS30 of Tn125.

Conclusions

Our study reveals the presence of CR-Ab of the sporadically reported ST600 harbouring the bla_NDM-1 gene, reported for the first time in Poland. Routine genomic surveillance should be implemented to monitor and reduce the transmission of circulating resistant A. baumannii clones.

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.