在天然MHC ii缺陷硬骨鱼中持续的T细胞非依赖性2型抗体反应。

IF 2.4 3区农林科学 Q1 FISHERIES

Developmental and comparative immunology Pub Date : 2025-01-30 DOI:10.1016/j.dci.2025.105330

Adrián López-Porras , Alexandra Jonsson , Shuo-Wang Qiao , Finn-Eirik Johansen

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引用次数: 0

摘要

众所周知，大西洋鳕鱼（Gadus morhua）在免疫后表现出较差的特异性抗体反应，尽管其天然IgM水平很高。与哺乳动物和几乎所有其他脊椎动物不同，鳕鱼缺乏T细胞依赖性（TD）抗体反应所需的MHC II-CD4轴。我们在哺乳动物中评估了鳕鱼对半抗原载体诱导T细胞非依赖性（TI）和TD反应的抗体反应。值得注意的是，鳕鱼产生了很强的半抗原特异性IgM水平，以对抗与TI-2抗原相关的半抗原ficoll或葡聚糖。特异性IgM水平在单次免疫后6-9周达到稳定水平，并在6个月内保持稳定。血清IgM的半衰期为4天，表明在此期间IgM持续产生。这些发现揭示了大西洋鳕鱼独特的免疫机制，增强了我们对脊椎动物免疫学的理解，并有助于开发新的水产养殖疫苗接种策略。

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Sustained T cell-independent type 2 antibody response in a naturally MHC II-deficient teleost fish

Atlantic cod (Gadus morhua) is well-known to show poor specific antibody responses after immunization, despite having high natural IgM levels. Unlike mammals and nearly all examined other vertebrates, cod lack the MHC II-CD4 axis required for T cell-dependent (TD) antibody responses. We evaluated the cod's antibody response to hapten carriers inducing T cell-independent (TI) and TD responses in mammals. Remarkably, cod generated strong hapten-specific IgM levels against hapten linked to the TI-2 antigens ficoll or dextran. Specific IgM levels plateaued 6–9 weeks after a single immunization and remained stable for six months. The serum half-life of IgM was 4 days, indicating continuous IgM production during this period. These findings reveal unique immune mechanisms in Atlantic cod, enhancing our understanding of vertebrate immunology and aiding the development of novel vaccination strategies in aquaculture.

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来源期刊

Developmental and comparative immunology 医学-动物学

CiteScore

6.20

自引率

6.90%

发文量

206

审稿时长

49 days

期刊介绍： Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.