核孔蛋白PNET1协调有丝分裂核孔复合体动力学,促进细胞快速分裂

IF 15.8 1区 生物学 Q1 PLANT SCIENCES
Yiling Fang, Yu Tang, Peiqiao Xie, Kendall Hsieh, Heejae Nam, Min Jia, Andres V. Reyes, Yuchen Liu, Shouling Xu, Xiaosa Xu, Yangnan Gu
{"title":"核孔蛋白PNET1协调有丝分裂核孔复合体动力学,促进细胞快速分裂","authors":"Yiling Fang, Yu Tang, Peiqiao Xie, Kendall Hsieh, Heejae Nam, Min Jia, Andres V. Reyes, Yuchen Liu, Shouling Xu, Xiaosa Xu, Yangnan Gu","doi":"10.1038/s41477-025-01908-y","DOIUrl":null,"url":null,"abstract":"The nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the nucleocytoplasmic shuttling of macromolecules. Here we report that Plant Nuclear Envelope Transmembrane 1 (PNET1), a transmembrane nucleoporin, is an adaptable NPC component that is mainly expressed in actively dividing cells. PNET1’s selective incorporation into the NPC is required for rapid cell growth in highly proliferative meristem and callus tissues in Arabidopsis. We demonstrate that the cell cycle-dependent phosphorylation of PNET1 coordinates mitotic disassembly and post-mitotic reassembly of NPCs during the cell cycle. PNET1 hyperphosphorylation disrupts its interaction with the NPC scaffold, facilitating NPC dismantling and nuclear membrane breakdown to trigger mitosis. In contrast, nascent, unphosphorylated PNET1 is incorporated into the nuclear pore membrane in the daughter cells, where it restores interactions with scaffolding nucleoporins for NPC reassembly. The expression of the human PNET1 homologue is required for and markedly upregulated during cancer cell growth, suggesting that PNET1 plays a conserved role in facilitating rapid cell division during open mitosis in highly proliferative tissues. The nuclear pore complex is a cornerstone of eukaryotic cell function. This study identifies a cell-type-specific nucleoporin that regulates mitotic disassembly and reassembly of the nuclear pore complex, serving as a critical mechanism to promote rapid cell division.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"295-308"},"PeriodicalIF":15.8000,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division\",\"authors\":\"Yiling Fang, Yu Tang, Peiqiao Xie, Kendall Hsieh, Heejae Nam, Min Jia, Andres V. Reyes, Yuchen Liu, Shouling Xu, Xiaosa Xu, Yangnan Gu\",\"doi\":\"10.1038/s41477-025-01908-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the nucleocytoplasmic shuttling of macromolecules. Here we report that Plant Nuclear Envelope Transmembrane 1 (PNET1), a transmembrane nucleoporin, is an adaptable NPC component that is mainly expressed in actively dividing cells. PNET1’s selective incorporation into the NPC is required for rapid cell growth in highly proliferative meristem and callus tissues in Arabidopsis. We demonstrate that the cell cycle-dependent phosphorylation of PNET1 coordinates mitotic disassembly and post-mitotic reassembly of NPCs during the cell cycle. PNET1 hyperphosphorylation disrupts its interaction with the NPC scaffold, facilitating NPC dismantling and nuclear membrane breakdown to trigger mitosis. In contrast, nascent, unphosphorylated PNET1 is incorporated into the nuclear pore membrane in the daughter cells, where it restores interactions with scaffolding nucleoporins for NPC reassembly. The expression of the human PNET1 homologue is required for and markedly upregulated during cancer cell growth, suggesting that PNET1 plays a conserved role in facilitating rapid cell division during open mitosis in highly proliferative tissues. The nuclear pore complex is a cornerstone of eukaryotic cell function. This study identifies a cell-type-specific nucleoporin that regulates mitotic disassembly and reassembly of the nuclear pore complex, serving as a critical mechanism to promote rapid cell division.\",\"PeriodicalId\":18904,\"journal\":{\"name\":\"Nature Plants\",\"volume\":\"11 2\",\"pages\":\"295-308\"},\"PeriodicalIF\":15.8000,\"publicationDate\":\"2025-01-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nature Plants\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.nature.com/articles/s41477-025-01908-y\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Plants","FirstCategoryId":"99","ListUrlMain":"https://www.nature.com/articles/s41477-025-01908-y","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

核孔复合体(NPC)是真核细胞功能的基石,协调大分子的核胞质穿梭。植物核膜跨膜1 (Plant Nuclear Envelope Transmembrane 1, PNET1)是一种跨膜核孔蛋白,是一种主要在活跃分裂细胞中表达的适应性NPC成分。PNET1选择性结合到NPC中是拟南芥高增殖分生组织和愈伤组织中细胞快速生长所必需的。我们证明PNET1的细胞周期依赖性磷酸化在细胞周期中协调NPCs的有丝分裂解体和有丝分裂后重组。PNET1的过度磷酸化破坏了其与NPC支架的相互作用,促进了NPC的拆除和核膜的破坏,从而触发有丝分裂。相反,未磷酸化的新生PNET1被整合到子细胞的核孔膜中,在那里它恢复与支架核孔蛋白的相互作用,用于NPC重组。人类PNET1同系物的表达在癌细胞生长过程中是必需的,并且在癌细胞生长过程中显著上调,这表明PNET1在高增殖组织开放有丝分裂过程中促进细胞快速分裂中起保守作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division

Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division

Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division
The nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the nucleocytoplasmic shuttling of macromolecules. Here we report that Plant Nuclear Envelope Transmembrane 1 (PNET1), a transmembrane nucleoporin, is an adaptable NPC component that is mainly expressed in actively dividing cells. PNET1’s selective incorporation into the NPC is required for rapid cell growth in highly proliferative meristem and callus tissues in Arabidopsis. We demonstrate that the cell cycle-dependent phosphorylation of PNET1 coordinates mitotic disassembly and post-mitotic reassembly of NPCs during the cell cycle. PNET1 hyperphosphorylation disrupts its interaction with the NPC scaffold, facilitating NPC dismantling and nuclear membrane breakdown to trigger mitosis. In contrast, nascent, unphosphorylated PNET1 is incorporated into the nuclear pore membrane in the daughter cells, where it restores interactions with scaffolding nucleoporins for NPC reassembly. The expression of the human PNET1 homologue is required for and markedly upregulated during cancer cell growth, suggesting that PNET1 plays a conserved role in facilitating rapid cell division during open mitosis in highly proliferative tissues. The nuclear pore complex is a cornerstone of eukaryotic cell function. This study identifies a cell-type-specific nucleoporin that regulates mitotic disassembly and reassembly of the nuclear pore complex, serving as a critical mechanism to promote rapid cell division.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Nature Plants
Nature Plants PLANT SCIENCES-
CiteScore
25.30
自引率
2.20%
发文量
196
期刊介绍: Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信