Denyss Guilcazo, Liseth Salinas, Cristina Chavez, Katherine Vasquez, Gabriela I Mendez, Lance B Price, Jay P Graham, Joseph N S Eisenberg, Gabriel Trueba
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Tracking blaCTX-M transmission through transposable elements in uropathogenic and commensal E. coli.
Aim: To investigate the nucleotide sequences associated with transposable elements carrying blaCTX-M allelic variants as potential markers for the transmission of antimicrobial resistance genes between domestic animals, humans and the environment.
Materials & methods: We conducted whole-genome sequencing and analyzed the nucleotide sequences of most abundant blaCTX-M allelic variants (blaCTX-M-27, blaCTX-M-55, and blaCTX-M-65) in commensal Escherichia coli (n = 20) from household members in Quito and uropathogenic E. coli (UPEC) (n = 149) isolated from nine clinics in Quito, Ecuador.
Results: The Ecuadorian commensal E. coli and UPEC displayed identical nucleotide sequences surrounding the blaCTX-M gene and the synteny was similar to those found in other parts of the world; however phylogenetic analysis indicated that the genetic environments in Ecuadorian isolates were unique.
Conclusion: These findings suggest that the nucleotide sequences flanking the blaCTX-M genes may be useful for resolving ARG transmission pathways, especially inter-regional analyses.
期刊介绍：
Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.
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