马尾藻乙醇提取物通过调节角化细胞HaCaT中FAK/Src/Akt/p38和Rac1信号通路促进伤口愈合

IF 2.1 Q3 PHARMACOLOGY & PHARMACY
Advances in Pharmacological and Pharmaceutical Sciences Pub Date : 2025-01-22 eCollection Date: 2025-01-01 DOI:10.1155/adpp/7198281
Furoida Moolsup, Wanida Sukketsiri, Wipawadee Sianglum, Jirawat Saetan, Nattakanwadee Khumpirapang, Supita Tanasawet
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引用次数: 0

摘要

最近,人们发现海藻提取物对皮肤有潜在的好处。因此,本研究旨在探讨褐藻马尾藻乙醇提取物(SPEE)对人皮肤角质形成细胞HaCaT细胞的植物化学分析、抗菌、抗氧化和伤口愈合特性及其可能的机制。结果表明,SPEE的主要活性成分为类黄酮、酚类和类胡萝卜素。HPLC图谱显示SPEE中存在c -藻蓝蛋白和岩藻聚糖。在伤口愈合实验中,SPEE显示出抗氧化能力,并在24和48 h显著减少伤口空间。western blot结果显示,SPEE(50和100 μg/mL)可提高FAK和Src磷酸化水平。此外,SPEE还上调Akt和p38 MAPK磷酸化,但不上调ERK1/2。SPEE增加了Rac1蛋白的表达。有趣的是,与对照组相比,暴露于SPEE后,透明质酸合成酶(HAS1和HAS2)以及I型胶原蛋白和弹性蛋白也显著上调。综上所述,我们的数据表明SPEE通过调节FAK/ src介导的Akt、p38 MAPK和Rac1信号通路促进皮肤伤口愈合。这些发现提示SPEE在皮肤伤口治疗中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sargassum plagiophyllum Ethanolic Extract Enhances Wound Healing by Modulating FAK/Src/Akt/p38 and Rac1 Signaling in Keratinocytes HaCaT Cells.

Recently, seaweed extracts have been found to have potential in skin benefits. This study, therefore, aimed to explore phytochemical analysis, antimicrobial, antioxidant, and wound healing properties of brown seaweed Sargassum plagiophyllym ethanolic extract (SPEE) on human skin keratinocyte HaCaT cells and the possible mechanism involved. Our results indicated that SPEE contained flavonoid, phenolic, and carotenoid as the major active constituents. The HPLC chromatogram revealed C-phycocyanin and fucoidan presented in SPEE. SPEE demonstrated the antioxidant capability and significantly reduced wound space at 24 and 48 h in wound-healing assay. Treatment with SPEE (50 and 100 μg/mL) increased FAK and Src phosphorylation in western blotting. Moreover, SPEE also upregulated Akt and p38 MAPK phosphorylation but not ERK1/2. SPEE increased Rac1 protein expression. Interestingly, hyaluronan synthase (HAS1 and HAS2) as well as collagen type I and elastin were also significantly upregulated when compared with the control upon exposure to SPEE. In conclusion, our data suggested that SPEE promotes cutaneous wound healing by regulating FAK/Src-mediated Akt, p38 MAPK, and Rac1 signaling. These findings suggest the potential use of SPEE for skin wound treatment.

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来源期刊
CiteScore
4.30
自引率
3.60%
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审稿时长
17 weeks
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