MOTS-c对肌肉细胞分化和纤维代谢的影响。

IF 2.5 Q3 CELL BIOLOGY
Natalia Leciejewska, Ewa Pruszyńska-Oszmałek, Paweł Kołodziejski, Dawid Szczepankiewicz, Leszek Nogowski, Maciej Sassek
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引用次数: 0

摘要

背景/目的:MOTS-c属于一组参与体内代谢过程的线粒体肽。自2015年发现以来,这种肽越来越受到关注,因为它有可能改善患有糖尿病或胰岛素抵抗的动物的代谢参数。MOTS-c参与肌肉代谢;然而,人们对其在纤维分化中的作用知之甚少。方法:我们以代表不同代谢类型肌纤维的C2C12和L6细胞系为研究对象,探讨MOTS-c对细胞过程的影响。研究方法为实时荧光定量PCR、Western blot和脂质积累测定。结果:值得注意的是,我们的研究显示,在10和100 nM剂量下,MOTS-c增加了C2C12细胞的存活率(pp结论:总之,我们的研究结果表明,MOTS-c的不同反应取决于肌肉纤维的类型,特别是在存活、细胞分化和脂质积累方面。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MOTS-c Impact on Muscle Cell Differentiation and Metabolism Across Fiber Types.

Background/aims: MOTS-c belongs to a group of mitochondrial peptides involved in metabolic processes in the body. This peptide has garnered increasing attention since its discovery in 2015 because of its potential to ameliorate metabolic parameters in animals with diabetes or insulin resistance. MOTS-c is involved in muscle metabolism; however, little is known about its role in fiber differentiation.

Methods: We conducted a study to explore the effect of MOTS-c on cellular processes using the C2C12 and L6 cell lines, representing different metabolic types of muscle fibers. The research methods were real-time PCR, Western blot, and lipid accumulation measurement.

Results: Notably, our investigations revealed that MOTS-c increased the survival of C2C12 cells at doses of 10 and 100 nM (p<0.01) and stimulated the phosphorylation of extracellular signal-regulated kinase within 5 min of incubation (p<0.05). Remarkably, these effects were not observed in L6 cells; however, both cell lines showed a reduced rate of proliferation. Furthermore, MOTS-c promotes the differentiation of C2C12 cells by increasing the expression of muscle regulatory factors, but it does not produce such an effect in L6 cells. Additionally, cells were treated with physiological concentrations of free fatty acids and MOTS-c, unveiling an augmentation in lipid accumulation observed in L6 cells and a decrease in lipid accumulation in C2C12 cells.

Conclusion: In conclusion, our findings have suggested a diverse response to MOTS-c depending on the type of muscle fibers, particularly in the domains of survival, cell differentiation, and lipid accumulation.

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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
86
审稿时长
1 months
期刊介绍: Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.
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