产妇肥胖与血液和初乳体液免疫改变有关。

IF 7.6 2区 医学 Q1 IMMUNOLOGY
Erick S. Sánchez-Salguero , Jessica Lakshmi Prieto-Chávez , Claudia Angélica García-Alonso , Anna-Maria Lampousi , Mario R. Alcorta-García , Víctor J. Lara-Diaz , Claudia N. López-Villaseñor , Marion E.G. Brunck
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引用次数: 0

摘要

孕产妇肥胖是一种在世界范围内日益流行的疾病,它与婴儿的不良结局相关。在这里,我们进行了一项观察性横断面研究,收集了37名体重指数在18.5-25或 > 30 kg/m2之间的母亲(分别为21名和16名母亲)的外周血和初乳样本。流式细胞术检测B淋巴细胞亚群。测定初乳驻留B细胞的IgG、IgA和IgM浓度以及抗体产生。总体而言,naïve B淋巴细胞是外周血中最丰富的亚型,而CD27-IgD-双阴性B细胞是初乳中最丰富的B淋巴细胞亚型。BMI为 > 30 kg/m2的母鼠初乳含有更多分泌IgG的初乳驻留B细胞,IgG更多,IgA更少。BMI为 > 30 kg/m2的母亲在妊娠晚期接种了辉瑞BioNTech二价疫苗(n = 8),与未接种疫苗的母亲相比,在任何组织类型中,与BMI为18.5-25 kg/m2的母亲(n = 7)相比,对SARS-CoV-2 RBD的IgA或IgG抗体反应均不高。在tdap疫苗接种后的初乳中也证实了类似的趋势。这是肥胖母亲初乳中B淋巴细胞亚群和抗体的首次表征。这项工作揭示了母亲肥胖作为初乳体液免疫成分的可能调节剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Maternal obesity associates with altered humoral immunity in blood and colostrum
Maternal obesity is a condition with increasing prevalence worldwide, that correlates with negative infant outcomes. Here we performed an observational cross-sectional study, where peripheral blood and colostrum samples from 37 mothers with BMI between 18.5–25 or > 30 kg/m2 (21 and 16 mothers, respectively) were collected 24–48 h postpartum. B lymphocyte subpopulations were investigated using flow cytometry. IgG, IgA, and IgM concentrations, and antibody production from colostrum-resident B cells were quantified. Overall, naïve B lymphocytes were the most abundant subtype in peripheral blood, while CD27-IgD- double-negative B cells were the most frequent in colostrum. The colostrum from mothers with BMI > 30 kg/m2 contained significantly more IgG-secreting colostrum-resident B cells, more total IgG, and less total IgA. Mothers with BMI > 30 kg/m2 who had been vaccinated with the Pfizer BioNTech bivalent vaccine during the third trimester of pregnancy (n = 8) did not show higher IgA or IgG antibody responses against SARS-CoV-2 RBD in either tissue types compared to unvaccinated mothers, contrasting with mother of BMI between 18.5–25 kg/m2 (n = 7). This is the first characterization of B lymphocyte subpopulations and antibodies in the colostrum of mothers with obesity. This work uncovers maternal obesity as a possible modifier of humoral immune components in colostrum.
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来源期刊
Mucosal Immunology
Mucosal Immunology 医学-免疫学
CiteScore
16.60
自引率
3.80%
发文量
100
审稿时长
12 days
期刊介绍: Mucosal Immunology, the official publication of the Society of Mucosal Immunology (SMI), serves as a forum for both basic and clinical scientists to discuss immunity and inflammation involving mucosal tissues. It covers gastrointestinal, pulmonary, nasopharyngeal, oral, ocular, and genitourinary immunology through original research articles, scholarly reviews, commentaries, editorials, and letters. The journal gives equal consideration to basic, translational, and clinical studies and also serves as a primary communication channel for the SMI governing board and its members, featuring society news, meeting announcements, policy discussions, and job/training opportunities advertisements.
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