- Book学术

发布求助

文献互助智能选刊最新文献

IF 5.8 3区环境科学与生态学 0 ENVIRONMENTAL SCIENCES

Environmental Science and Pollution Research Pub Date : 2025-01-28 DOI:10.1007/s11356-025-35988-2

Emília Marques Brovini, Marcela Dias, Amanda Teodoro, Elayne Machado, Letícia Santos, Cintia Leal, Sérgio Francisco de Aquino, Juliana Calábria de Araújo

{"title":"Effects of UVC doses on the removal of antimicrobial resistance elements from secondary treated sewage.","authors":"Emília Marques Brovini, Marcela Dias, Amanda Teodoro, Elayne Machado, Letícia Santos, Cintia Leal, Sérgio Francisco de Aquino, Juliana Calábria de Araújo","doi":"10.1007/s11356-025-35988-2","DOIUrl":null,"url":null,"abstract":"Wastewater treatment plants (WWTPs) currently face major challenges toward the removal of microcontaminants and/or microbial matrices and consequently play an important role in the potential dissemination of biological resistance in freshwater. The ultraviolet (UV) system is a tertiary treatment strategy increasingly applied worldwide, although many studies have shown that disinfected effluent can still contain antibiotic-resistant bacteria and resistance genes. Therefore, to better understand the effects of UV radiation doses on the removal of all resistance elements (antibiotics, antibiotic-resistant bacteria, and antibiotic resistance genes), the present study was designed using a pilot-scale photoreactor. The UV doses could be varied to investigate whether there is an optimal UV dose capable of removing all resistance elements and also if the UV dose frequently applied in full-scale systems is able to reduce the resistance elements. The effect of different UV doses (A, 0-10 mJ/cm2; B, 10-15 mJ/cm2; and C, > 15 mJ/cm2) in a pilot-scale photoreactor on the removal of antibiotics, antibiotic-resistant bacteria, and genes from the effluent of a UASB reactor followed by a biological trickling filter system (UASB-TF) fed with real sanitary sewage was investigated. Samples of influent and effluent from the UVC photoreactor were collected, and the concentration levels of norfloxacin (NOR), ciprofloxacin (CIP), and levofloxacin (LEV) were assessed. The qnrB, sul1, ermB, integron-integrase (intI1), and 16S rRNA genes, total heterotrophic bacteria (THB), and bacterial resistance to azithromycin and sulfamethoxazole were also investigated. Results indicated that LEV and intI1 were found in the highest median concentrations in the photoreactor influent. Although most antibiotics (NOR and CIP) and ARGs (intI1, 16S rRNA, and qnrB) were apparently better removed with the highest UV dose (> 15 mJ/cm2) applied, except for LEV, sul1, and ermB genes, the Kruskal-Wallis test reported no significant difference between low and high doses. ARB removal (from 80 to 100%) was observed at all UV doses. Principal component analysis (PCA) suggested a clear pattern of pollutant groups, i.e., antibiotics, ARG, and ARB, which exhibited low (median of 8-16%), medium (37-96%), and high (> 97%) removal percentages, respectively. These results demonstrated that UVC photoreactors can be an alternative to complement biological treatment in sewage treatment plants at the dose normally applied in full-scale WWTPs (> 15 mJ/cm2). However, there was no optimal single dose capable of removing all the resistance elements investigated.","PeriodicalId":545,"journal":{"name":"Environmental Science and Pollution Research","volume":" ","pages":""},"PeriodicalIF":5.8000,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Science and Pollution Research","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s11356-025-35988-2","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"0","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

目前，污水处理厂（WWTPs）在去除微污染物和/或微生物基质方面面临着重大挑战，因此在淡水生物抗药性的潜在传播中扮演着重要角色。紫外线（UV）系统是全球范围内应用日益广泛的一种三级处理策略，但许多研究表明，消毒后的污水中仍可能含有抗生素耐药细菌和耐药基因。因此，为了更好地了解紫外线辐射剂量对去除所有抗药性元素（抗生素、抗生素耐药菌和抗生素耐药基因）的影响，本研究设计了一个中试规模的光反应器。紫外线剂量可以改变，以研究是否存在能够去除所有抗药性元素的最佳紫外线剂量，以及在大规模系统中经常使用的紫外线剂量是否能够减少抗药性元素。研究了中试规模的光反应器中不同紫外线剂量（A，0-10 mJ/cm2；B，10-15 mJ/cm2；C，> 15 mJ/cm2）对去除 UASB 反应器和生物滴滤系统（UASB-TF）流出物中抗生素、抗生素耐药菌和基因的影响。收集了紫外线光反应器的进水和出水样本，并评估了诺氟沙星（NOR）、环丙沙星（CIP）和左氧氟沙星（LEV）的浓度水平。此外，还研究了 qnrB、sul1、ermB、整合酶（intI1）和 16S rRNA 基因、异养菌总数（THB）以及细菌对阿奇霉素和磺胺甲噁唑的耐药性。结果表明，LEV 和 intI1 在光反应器进水中的浓度中位数最高。虽然大多数抗生素（NOR 和 CIP）和 ARGs（intI1、16S rRNA 和 qnrB）在使用最高紫外线剂量（> 15 mJ/cm2）时去除效果更好，但除了 LEV、sul1 和 ermB 基因外，Kruskal-Wallis 检验表明低剂量和高剂量之间没有显著差异。在所有紫外线剂量下都观察到了 ARB 的去除率（从 80% 到 100% 不等）。主成分分析（PCA）表明，抗生素、ARG 和 ARB 等污染物组别具有明显的模式，其去除率分别为低（中位数为 8-16%）、中（37-96%）和高（> 97%）。这些结果表明，紫外线光反应器可以作为污水处理厂生物处理的补充，其剂量通常为全规模污水处理厂所使用的剂量（> 15 mJ/cm2）。不过，没有一种最佳的单一剂量能够去除所有被调查的阻力元素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Effects of UVC doses on the removal of antimicrobial resistance elements from secondary treated sewage.

Wastewater treatment plants (WWTPs) currently face major challenges toward the removal of microcontaminants and/or microbial matrices and consequently play an important role in the potential dissemination of biological resistance in freshwater. The ultraviolet (UV) system is a tertiary treatment strategy increasingly applied worldwide, although many studies have shown that disinfected effluent can still contain antibiotic-resistant bacteria and resistance genes. Therefore, to better understand the effects of UV radiation doses on the removal of all resistance elements (antibiotics, antibiotic-resistant bacteria, and antibiotic resistance genes), the present study was designed using a pilot-scale photoreactor. The UV doses could be varied to investigate whether there is an optimal UV dose capable of removing all resistance elements and also if the UV dose frequently applied in full-scale systems is able to reduce the resistance elements. The effect of different UV doses (A, 0-10 mJ/cm²; B, 10-15 mJ/cm²; and C, > 15 mJ/cm²) in a pilot-scale photoreactor on the removal of antibiotics, antibiotic-resistant bacteria, and genes from the effluent of a UASB reactor followed by a biological trickling filter system (UASB-TF) fed with real sanitary sewage was investigated. Samples of influent and effluent from the UVC photoreactor were collected, and the concentration levels of norfloxacin (NOR), ciprofloxacin (CIP), and levofloxacin (LEV) were assessed. The qnrB, sul1, ermB, integron-integrase (intI1), and 16S rRNA genes, total heterotrophic bacteria (THB), and bacterial resistance to azithromycin and sulfamethoxazole were also investigated. Results indicated that LEV and intI1 were found in the highest median concentrations in the photoreactor influent. Although most antibiotics (NOR and CIP) and ARGs (intI1, 16S rRNA, and qnrB) were apparently better removed with the highest UV dose (> 15 mJ/cm²) applied, except for LEV, sul1, and ermB genes, the Kruskal-Wallis test reported no significant difference between low and high doses. ARB removal (from 80 to 100%) was observed at all UV doses. Principal component analysis (PCA) suggested a clear pattern of pollutant groups, i.e., antibiotics, ARG, and ARB, which exhibited low (median of 8-16%), medium (37-96%), and high (> 97%) removal percentages, respectively. These results demonstrated that UVC photoreactors can be an alternative to complement biological treatment in sewage treatment plants at the dose normally applied in full-scale WWTPs (> 15 mJ/cm²). However, there was no optimal single dose capable of removing all the resistance elements investigated.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Environmental Science and Pollution Research 环境科学-环境科学

CiteScore

8.70

自引率

17.20%

发文量

6549

审稿时长

3.8 months

期刊介绍： Environmental Science and Pollution Research (ESPR) serves the international community in all areas of Environmental Science and related subjects with emphasis on chemical compounds. This includes: - Terrestrial Biology and Ecology - Aquatic Biology and Ecology - Atmospheric Chemistry - Environmental Microbiology/Biobased Energy Sources - Phytoremediation and Ecosystem Restoration - Environmental Analyses and Monitoring - Assessment of Risks and Interactions of Pollutants in the Environment - Conservation Biology and Sustainable Agriculture - Impact of Chemicals/Pollutants on Human and Animal Health It reports from a broad interdisciplinary outlook.