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IF 5.4 2区生物学 Q1 PLANT SCIENCES

Physiologia plantarum Pub Date : 2025-01-01 DOI:10.1111/ppl.70063

Yilin Di, Yiqin Cao, Dandan Peng, Ying Liu, Zhou Li

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引用次数: 0

摘要

GAD1 基因编码谷氨酸脱羧酶，它是内源性γ-氨基丁酸（GABA）生物合成的限速酶，但 GAD1 在植物中调控镉（Cd）耐受性的潜在作用有待进一步阐明。本研究旨在调查匍匐翦股颖（Agrostis stolonifera）和转基因酵母（Saccharomyces cerevisiae）或拟南芥过表达 AsGAD1 的耐镉性。与对镉敏感的W66569相比，耐镉匍匐翦股颖LOFTSL-93在镉胁迫下叶片和根部积累了更多的内源GABA，这与AsGAD1的显著上调有关。AsGAD1 的过表达显著增强了酵母或大连蝙蝠蛾对镉的耐受性，这与内源 GABA 含量的提高、低氧化损伤、高细胞膜稳定性和光化学效率有关。与野生型相比，过表达 AsGAD1 的植株或 atgad1 突变体通过下调或上调 AtNRAMP1/2/3/4/5 和 AtZIP1/2 的转录本水平，使叶片和根中的镉含量显著降低或升高。此外，过表达 AsGAD1 能显著上调 AtHMA1/3 的转录水平，从而更好地将镉从叶绿体分区到细胞质，再分区到液泡。AsGAD1 的过表达还诱导了 AsMT1A/1B/1C/2/3、AsGSH1/2 和 AsPCS1/2 的表达，表明细胞质和液泡中的镉螯合能力更强，能更好地进行镉解毒。因此，AsGAD1调控的镉解毒机制可能与镉的吸收、转运和螯合有关。此外，AsGAD1的过表达还提高了脂质含量（PC、PG和DGDG）以及DGDG/MGDG和PC/PG的比例，这有利于镉胁迫下膜的稳定性和功能性。这些发现为了解 GAD1 在植物耐镉过程中的调控作用提供了新的视角。

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AsGAD1 cloned from creeping bentgrass modulates cadmium tolerance of Arabidopsis thaliana by remodelling membrane lipids and cadmium uptake, transport and chelation.

The gene GAD1 encodes a glutamate decarboxylase, which is a rate-limiting enzyme for the biosynthesis of endogenous γ-aminobutyrate acid (GABA), but a potential role of GAD1 in regulating cadmium (Cd) tolerance needs to be further elucidated in plants. The objective of this study was to investigate Cd tolerance of creeping bentgrass (Agrostis stolonifera) and transgenic yeast (Saccharomyces cerevisiae) or Arabidopsis thaliana overexpressing AsGAD1. The Cd-tolerant creeping bentgrass cultivar LOFTSL-93 accumulated more endogenous GABA in relation to a significant upregulation of AsGAD1 in leaf and root than the Cd-sensitive W66569 in response to Cd stress. The overexpression of AsGAD1 significantly enhanced Cd tolerance of yeast or A. thaliana associated with improved endogenous GABA content, low oxidative damage, and high cell membrane stability and photochemical efficiency. Compared with wild type, AsGAD1-overexpressing plants or the atgad1 mutant maintained significantly lower or higher Cd content in leaf and root by down-regulating or up-regulating transcript levels of AtNRAMP1/2/3/4/5 and AtZIP1/2, respectively. Moreover, overexpression of AsGAD1 significantly up-regulated transcript levels of AtHMA1/3, contributing to better Cd compartmentalization from chloroplast into cytoplasm and then into vacuoles. AsGAD1 overexpression also induced expressions of AsMT1A/1B/1C/2/3, AsGSH1/2, and AsPCS1/2, indicating better capacity of Cd chelation in cytosol and vacuoles for Cd detoxification. Hence, AsGAD1-regulated detoxification mechanism of Cd could be related to Cd uptake, transport, and chelation. In addition, lipid contents (PC, PG, and DGDG) and the DGDG/MGDG and PC/PG ratios were improved by the AsGAD1 overexpression, which favors membrane stability and functionality under Cd stress. These findings provide new insight into the regulatory role of GAD1 in Cd tolerance in plants.

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来源期刊

Physiologia plantarum 生物-植物科学

CiteScore

11.00

自引率

3.10%

发文量

224

审稿时长

3.9 months

期刊介绍： Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.