碳化羟基磷灰石-他汀纳米乳对正畸复发大鼠acp5和Runx-2表达的影响。

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-01-01 DOI:10.1016/j.jobcr.2024.12.013

Ananto Ali Alhasyimi , Niswati Fathmah Rosyida , Ika Dewi Ana

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引用次数: 0

摘要

背景：固位是正畸治疗的重要组成部分。在过去的二十年里，许多生物治疗已经发展到减轻正畸复发。药物骨调节是减轻复发的可行方法。本研究的目的是确定给予碳化羟基磷灰石-他汀纳米乳（cha -他汀）是否会增加实验性正畸复发大鼠Runx-2表达并降低Acp - 5表达。材料与方法：48只大鼠分为对照组、CHA组、他汀类药物组、CHA-他汀类药物组，每组12只。通过闭合线圈弹簧将上颌第一磨牙与上颌切牙连接，施加30 g内侧牵引7天。采用CHA、他汀水凝胶和CHA-他汀纳米乳，每3 d静脉注射一次，连续7 d。器械的移除促进了复发的发生。免疫组织化学法检测acp5和Runx-2的表达。结果：在复发运动的第7天和第14天，与其他组相比，cha -他汀组的acp5阳性细胞明显减少(p p结论：cha -他汀纳米乳增加了Runx-2的表达，降低了acp5的表达，从而可能预防正畸大鼠的复发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats

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Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats

Background

Retention is an essential element of orthodontic treatment. In the past two decades, numerous biological treatments have been developed to alleviate orthodontic relapse. Pharmacologic bone modulation is a viable approach to mitigate relapse. The aim of this investigation was to determine whether administering nanoemulsion carbonated hydroxyapatite-statin (CHA-statin) would increase Runx-2 expression and decrease Acp 5 expression in rats experiencing experimental orthodontic relapse.

Materials & methods

Forty-eight rats (n = 48) were assigned to four groups: control, CHA, statin, and CHA-statin, with 12 rats in each group. A 30 g mesial traction was applied for 7 days via a closed-coil spring that connected the first maxillary molar to the maxillary incisor. To maintain the moved teeth, CHA, statin hydrogel, and nanoemulsion CHA-statin were administered intrasulcularly every 3 days for a period of 7 days. The removal of the devices facilitated the occurrence of relapse. The expression of Acp 5 and Runx-2 was evaluated using the immunohistochemistry method. The collected data were evaluated using analysis of variance and post hoc tests, with p < 0.05.

Results

A significant reduction in Acp 5-positive cells in the CHA-statin group was observed on days 7 and 14 of relapse movement compared with the other groups (p < 0.05), whereas the average Runx-2 expressions in the CHA-statin groups were significantly higher than in the other groups on days 1, 7, and 14 during the relapse phase (p < 0.05).

Conclusion

The nanoemulsion CHA-statin increased Runx-2 expression and decreased Acp 5 expression, thereby potentially preventing orthodontic relapse in rats.

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.