发育中的斑马鱼和成年斑马鱼的树突状细胞起源于不同的起源，并表现出不同的细胞依赖性。

IF 3.7 2区生物学 Q1 DEVELOPMENTAL BIOLOGY

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI:10.1242/dev.204410

Guanzhen Lin, Youqi Wang, Thi Giang Pham, Zilong Wen

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引用次数: 0

摘要

树突状细胞（dc）是免疫系统的关键细胞成分，在先天免疫和获得性免疫中发挥重要作用。在哺乳动物中，一般认为dc完全起源于造血干细胞（hsc）。利用时空分辨的命运图谱系统，我们发现斑马鱼的dc有两个来源：背主动脉内皮衍生的造血祖细胞（EHPs）和造血干细胞。ehp衍生的树突状细胞出现较早，主要在幼虫期定殖于发育中的胸腺，在幼年期逐渐减少。相比之下，hsc衍生的dc出现较晚，可以在幼虫后期到成年期的不同组织中生长。我们进一步证明EHP和hsc衍生的DC对fms样酪氨酸激酶3 （Flt3）的依赖性不同，Flt3是一种关键受体酪氨酸激酶，对哺乳动物DC的发育至关重要。我们的研究揭示了斑马鱼DC发育的两个不同波的存在，每个波都有独特的起源和发育控制。

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Dendritic cells in developing and adult zebrafish arise from different origins and display distinct flt3 dependencies.

Dendritic cells (DCs) are key cellular components of the immune system and perform crucial functions in innate and acquired immunity. In mammals, it is generally believed that DCs originate exclusively from hematopoietic stem cells (HSCs). Using a temporal-spatial resolved fate-mapping system, here we show that, in zebrafish, DCs arise from two sources: dorsal aorta-born endothelium-derived hematopoietic progenitors (EHPs) and HSCs. The EHP-derived DCs emerge early, predominantly colonizing the developing thymus during larval stages and diminishing by juvenile stages. In contrast, HSC-derived DCs emerge later and can populate different tissues from late larval stages to adulthood. We further document that the EHP- and HSC-derived DCs display different dependencies on Fms-like tyrosine kinase 3 (Flt3), a pivotal receptor tyrosine kinase crucial for DC development in mammals. Our study reveals the presence of two distinct waves of DC development in zebrafish, each with unique origins and developmental controls.

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来源期刊

Development 生物-发育生物学

CiteScore

6.70

自引率

4.30%

发文量

433

审稿时长

3 months

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