通过细胞器特异性DNA组装策略对线粒体中非编码rna的空间选择性成像

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-01-27 DOI:10.1021/acs.nanolett.4c05559

Bo Wu, Jian Zhao, Fangzhi Yu, Lele Li, Yuliang Zhao

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引用次数: 0

摘要

特定细胞器中非编码rna （ncRNAs）的精确成像允许在亚细胞水平解码其功能，但缺乏先进的工具。在这里，我们提出了一种基于DNA的纳米生物技术，用于通过细胞器特异性DNA组装策略对线粒体中的ncRNA（例如microRNA (miRNA)）进行空间选择性成像。靶miRNA启动的DNA发夹组装被阻断脚位介导的链位移反应所抑制，但可以被线粒体编码的核糖体RNA （rRNA）独家激活进行杂交链反应，从而实现对miRNA成像的空间控制。我们证明了条件控制DNA组装技术允许最小化非特异性激活，从而提高了miRNA检测的空间精度。此外，该策略适用于可视化其他ncrna，如线粒体中的长链非编码rna，突出了该方法的普遍性。总的来说，这项工作为ncrna的空间选择性成像和研究细胞器定位RNA的功能提供了一个有用的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Spatioselective Imaging of Noncoding RNAs in Mitochondria via an Organelle-Specific DNA Assembly Strategy

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Spatioselective Imaging of Noncoding RNAs in Mitochondria via an Organelle-Specific DNA Assembly Strategy

Precise imaging of noncoding RNAs (ncRNAs) in specific organelles allows decoding of their functions at subcellular level but lacks advanced tools. Here we present a DNA-based nanobiotechnology for spatially selective imaging of ncRNA (e.g., microRNA (miRNA)) in mitochondria via an organelle-specific DNA assembly strategy. The target miRNA-initiated assembly of DNA hairpins is inhibited by the block of toehold-mediated strand displacement reaction but can be exclusively activated by a mitochondria-encoded ribosomal RNA (rRNA) for hybridization chain reaction, enabling spatial control over miRNA imaging. We demonstrate that the conditionally controlled DNA assembly technology allows for minimization of nonspecific activation and thus improves the spatial precision of miRNA detection. In addition, the strategy is adaptable to visualizing other ncRNAs such as long noncoding RNAs in mitochondria, highlighting the universality of the approach. Overall, this work provides a useful tool for spatially selective imaging of ncRNAs and investigating the functions of organelle-located RNA.

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.