IF 3.8 3区 医学 Q2 VIROLOGY
Viruses-Basel Pub Date : 2025-01-15 DOI:10.3390/v17010115
Lika Leshkasheli, Ia Kusradze, Darejan Bolkvadze, Lia Askilashvili, Maria Chichashvili, Giorgi Tsertsvadze, Elisabed Zaldastanishvili
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引用次数: 0

摘要

抗生素耐药性在全球范围内迅速蔓延,正迅速成为人类医疗保健领域日益严重的问题。包括肺炎克雷伯氏菌在内的许多革兰氏阴性细菌病原体对非抗生素抗菌剂的需求量很大。克雷伯氏菌靶向噬菌体是最有前途的替代疗法之一。它们已成功应用于多个病例,预计未来对抗克雷伯氏菌噬菌体的需求只会增加。这一前景凸显了对特性良好的治疗噬菌体的需求。在这项研究中,我们描述了一种肺炎克雷伯氏菌噬菌体,它也能感染氧合克雷伯氏菌菌株。在此,我们从生物学和基因特性的角度描述了噬菌体 M198 的特征。由于在一些噬菌体治疗案例中,噬菌体会与抗生素联合使用,因此我们在此还筛选了噬菌体 M198 与六种不同抗生素联合使用可能产生的协同效应。我们发现,噬菌体 M198 对临床分离株具有良好的溶菌活性;它没有任何温带生活方式的迹象,而且在与一些治疗相关的抗生素联合使用时具有协同增效的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Klebsiella pneumoniae Phage M198 and Its Therapeutic Potential.

The rapid worldwide spread of antibiotic resistance is quickly becoming an increasingly concerning problem for human healthcare. Non-antibiotic antibacterial agents are in high demand for many Gram-negative bacterial pathogens, including Klebsiella pneumoniae. Klebsiella-targeting phages are among the most promising alternative therapy options. They have already been successfully applied in a number of cases, and it is expected that the need for anti-Klebsiella phages will only increase in the future. This prospect highlights the need for well-characterized therapeutic phages. In this work, we describe a K. pneumoniae phage, which also infects strains of Klebsiella oxytoca. Here, we characterize phage M198 in terms of its biological and genetic properties. Since in some phage therapy cases, phages are administered in combination with antibiotics, here, we also screen for possible synergistic effects of combining phage M198 with six different antibiotics. We found that phage M198 has good lytic activity against clinical isolates; it does not have any indications of a temperate lifestyle, and it has synergistic potential when combined with some therapeutically relevant antibiotics.

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来源期刊
Viruses-Basel
Viruses-Basel VIROLOGY-
CiteScore
7.30
自引率
12.80%
发文量
2445
审稿时长
1 months
期刊介绍: Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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