阿尔及利亚东北部古尔马地区白蛉沙蝇和犬中利什曼原虫的发生。

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-01-01 DOI:10.1016/j.vprsr.2024.101176

Ilham Ferdes , Maria Stefania Latrofa , Livia Perles , Marcos Antonio Bezerra-Santos , Bachir Medrouh , Meryem Benmarce , Karim Ouachek , Ahcène Hakem , Ismail Lafri , Domenico Otranto

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引用次数: 0

摘要

利什曼原虫是沙蝇传播的寄生原生动物，分布在世界各地，可能严重影响狗以及包括人类在内的其他哺乳动物的健康和福利。阿尔及利亚是受影响最严重的国家之一，在人类和狗身上发现了几例婴儿利什曼原虫感染病例。在此，我们评估了阿尔及利亚东北部Guelma地区白蛉和犬中利什曼原虫的发生情况。2022年7月至9月采集沙蝇，2023年9月至2024年2月采集犬利什曼病（CanL）。此外，为了解人间感染的风险，报告了2012年至2023年该区域记录的利什曼病病例的回顾性数据。采用定量PCR方法对沙蝇和犬血标本进行利什曼原虫检测，对犬血清标本进行抗利什曼原虫检测。免疫荧光抗体试验（IFAT）检测婴儿抗体。在1478只沙蝇中(雌性318只；共鉴定出papatasi Phlebotomus、perfiliewi Ph.、sergenti Ph.、perniciosus Ph.、longicuspis Ph.、ariasi Ph.和minuta Sergentomyia 7种。在perniciosus博士、perfiliewi博士、papatasi博士和sergenti博士中检出利什曼原虫（3.7%）；n = 10)。总体血清阳性率为58.2%，其中1.6%的狗血液样本在分子筛选中呈婴儿乳杆菌阳性。不同危险因素的多因素分析显示，CanL血清阳性与犬龄（100 ~ 1岁）（OR = 8.35, 95% CI: 3.43 ~ 23.93）、秋季季节（OR = 2.95, 95% CI: 1.33 ~ 8.62）和未施用杀虫剂（OR = 6.69, 95% CI: 2.61 ~ 22.02）密切相关。本文共记录了71例皮肤利什曼病和内脏利什曼病。提出的数据揭示了CanL在Guelma地区的发生，主张在阿尔及利亚的这部分地区实施控制规划措施。

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Occurrence of Leishmania spp. in phlebotomine sand flies and dogs in Guelma region, North-eastern Algeria

Leishmania spp. are sand fly-borne parasitic protozoa of worldwide distribution that may severely affect the health and welfare of dogs as well as of other mammalian species, including humans. Algeria is among the most affected countries, counting several cases of Leishmania infantum infection in humans and dogs. Here, we assessed the occurrence of Leishmania species in both phlebotomine sand fly and dogs in the Guelma region, Northeast of Algeria. Sand flies were collected from July to September 2022, followed by a survey of canine leishmaniasis (CanL) from September 2023 to February 2024. Additionally, to understand the risk of human infection a retrospective data on cases of leishmaniasis recorded in the region from 2012 to 2023 were reported. Sand fly specimens and canine blood samples were tested by quantitative PCR (qPCR) for Leishmania spp., while dog serum samples were processed for anti-L. infantum antibodies detection by immunofluorescent antibody test (IFAT). Of the 1478 sand flies (n = 318 females; n = 1160 males), seven species were identified (i.e., Phlebotomus papatasi, Ph. perfiliewi, Ph. sergenti, Ph. perniciosus, Ph. longicuspis, Ph. ariasi, and Sergentomyia minuta). Leishmania spp. was detected in Ph. perniciosus, Ph. perfiliewi, Ph. papatasi, and Ph. sergenti (i.e., 3.7 %; n = 10). The overall seroprevalence rate was 58.2 %, with 1.6 % of dog blood samples positive for L. infantum at molecular screening. Multivariate analysis of the different risk factors revealed that CanL seropositivity was strongly related to dog age (> 1 year) (OR = 8.35, 95 % CI: 3.43–23.93), the autumn season (OR = 2.95, 95 % CI: 1.33–8.62), and lack of insecticide application (OR = 6.69, 95 % CI: 2.61–22.02). A total of 71 human cases of cutaneous (CL) and visceral (VL) leishmaniasis were recorded. Data presented reveal the occurrence of CanL in Guelma region, advocating for control program measures to be implemented in this part of Algeria.

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).