使用SeahorseXFe24分析仪实时评估游离小鼠视网膜光感受器能量代谢的方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-03-21 Epub Date: 2025-01-23 DOI:10.1016/j.xpro.2025.103595

Maria Yera, Joshua J Wang, Sarah X Zhang

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引用次数: 0

摘要

视网膜代谢缺陷与各种视网膜疾病的发生和发展有关。在此，我们提供了一个方案来测量生物能量在分离的小鼠视网膜光感受器。我们概述了从成年小鼠中获得形态完整和活的光感受器细胞的详细说明，并使用SeahorseXFe24分析仪准备细胞进行代谢分析。该方案允许实时评估线粒体呼吸和糖酵解视网膜光感受器在小鼠模型中对遗传修饰或病理损伤的反应。

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Protocol for real-time assessment of energy metabolism in dissociated mouse retinal photoreceptors using a SeahorseXFe24 analyzer.

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Protocol for real-time assessment of energy metabolism in dissociated mouse retinal photoreceptors using a SeahorseXFe24 analyzer.

Defects in retinal metabolism have been linked to the onset and progression of various retinal diseases. Herein, we provide a protocol for measuring bioenergetics in dissociated mouse retinal photoreceptors. We outline detailed instructions for obtaining morphologically intact and viable photoreceptor cells from adult mice and preparing the cells for metabolic analysis using a SeahorseXFe24 analyzer. This protocol allows a real-time assessment of mitochondrial respiration and glycolysis in retinal photoreceptors in response to genetic modifications or pathological insults in mouse models.

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