{"title":"肉鸡源禽致病性与肠外致病性大肠杆菌分离株致病性因子的比较。","authors":"S Sariçam İnce, A Ünal, M Akan","doi":"10.1080/00071668.2025.2451242","DOIUrl":null,"url":null,"abstract":"<p><p>1. <i>E. coli</i> is an opportunist pathogen of animals, including food-producing ones and humans. Chickens may be a notable source of pathogenic and antimicrobial resistant <i>E. coli</i> for transmission to humans.2. This study compared virulence-associated genes (VGs) and antimicrobial resistance (AMR) in avian pathogenic <i>E. coli</i> (APEC) and extraintestinal pathogenic E. coli (ExPEC) isolates from broiler chickens, specifically APEC isolates in liver samples (<i>n</i> = 78) and ExPEC or non-ExPEC isolates in litter samples (<i>n</i> = 34). Virulence was evaluated by PCR for <i>feoB, hlyF, iroN, iss, iutA</i> and <i>ompT</i> genes, while AMR was evaluated by using antimicrobials from seven classes and detecting <i>bla<sub>SHV</sub>, bla<sub>TEM</sub>, bla<sub>OXA</sub>, qnrB, stcM, mrc1, mrc2, sul1</i> and <i>tetA</i> genes.3. The APEC isolates were found in 100% of livers, while ExPEC and non-ExPEC isolates were found in 44% and 56% of the litter samples. The predominant VG was <i>feoB</i> (100%), followed by <i>ompT</i> (63%), <i>iutA</i> (60%), <i>iss</i> (58%) and <i>hlyF</i> (43%). Surprisingly, <i>iroN, omp T</i> and <i>iutA</i> had higher prevalences in APEC isolates (85%, 96% and 96%, respectively) than in ExPEC isolates (73%, 87% and 73%, respectively) and non-ExPEC isolates (0% for all). The presence of all VG in 33% of isolates indicated high pathogenicity.4. The isolates were phenotypically resistant to ampicillin (93%), ceftazidime (72%) and nalidixic acid (82%). All APEC and ExPEC isolates (100%) were multidrug resistant (MDR), while 63% of non-ExPEC isolates were MDR. Genotypic AMR testing revealed that 53% and 52% of all isolates had <i>stcM</i> and <i>tetA</i>, respectively. No isolate was positive for <i>bla<sub>SHV</sub>, bla<sub>OXA</sub>, mrc1</i> or <i>mrc2</i>, which suggested the benefits of colistin for treating carbapenem-resistant enteric pathogens, due to the high resistance detected to meropenem (47%).5. Given the potential pathogenicity of <i>E. coli</i> isolates, improving biosecurity practices in chicken flocks should be prioritised to eliminate transmission to humans through the food chain.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-8"},"PeriodicalIF":1.6000,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of pathogenicity factors of avian pathogenic and extraintestinal pathogenic <i>Escherichia coli</i> isolates originating from broiler chickens.\",\"authors\":\"S Sariçam İnce, A Ünal, M Akan\",\"doi\":\"10.1080/00071668.2025.2451242\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>1. <i>E. coli</i> is an opportunist pathogen of animals, including food-producing ones and humans. Chickens may be a notable source of pathogenic and antimicrobial resistant <i>E. coli</i> for transmission to humans.2. This study compared virulence-associated genes (VGs) and antimicrobial resistance (AMR) in avian pathogenic <i>E. coli</i> (APEC) and extraintestinal pathogenic E. coli (ExPEC) isolates from broiler chickens, specifically APEC isolates in liver samples (<i>n</i> = 78) and ExPEC or non-ExPEC isolates in litter samples (<i>n</i> = 34). Virulence was evaluated by PCR for <i>feoB, hlyF, iroN, iss, iutA</i> and <i>ompT</i> genes, while AMR was evaluated by using antimicrobials from seven classes and detecting <i>bla<sub>SHV</sub>, bla<sub>TEM</sub>, bla<sub>OXA</sub>, qnrB, stcM, mrc1, mrc2, sul1</i> and <i>tetA</i> genes.3. The APEC isolates were found in 100% of livers, while ExPEC and non-ExPEC isolates were found in 44% and 56% of the litter samples. The predominant VG was <i>feoB</i> (100%), followed by <i>ompT</i> (63%), <i>iutA</i> (60%), <i>iss</i> (58%) and <i>hlyF</i> (43%). Surprisingly, <i>iroN, omp T</i> and <i>iutA</i> had higher prevalences in APEC isolates (85%, 96% and 96%, respectively) than in ExPEC isolates (73%, 87% and 73%, respectively) and non-ExPEC isolates (0% for all). The presence of all VG in 33% of isolates indicated high pathogenicity.4. The isolates were phenotypically resistant to ampicillin (93%), ceftazidime (72%) and nalidixic acid (82%). All APEC and ExPEC isolates (100%) were multidrug resistant (MDR), while 63% of non-ExPEC isolates were MDR. Genotypic AMR testing revealed that 53% and 52% of all isolates had <i>stcM</i> and <i>tetA</i>, respectively. No isolate was positive for <i>bla<sub>SHV</sub>, bla<sub>OXA</sub>, mrc1</i> or <i>mrc2</i>, which suggested the benefits of colistin for treating carbapenem-resistant enteric pathogens, due to the high resistance detected to meropenem (47%).5. Given the potential pathogenicity of <i>E. coli</i> isolates, improving biosecurity practices in chicken flocks should be prioritised to eliminate transmission to humans through the food chain.</p>\",\"PeriodicalId\":9322,\"journal\":{\"name\":\"British Poultry Science\",\"volume\":\" \",\"pages\":\"1-8\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-01-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"British Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/00071668.2025.2451242\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"British Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/00071668.2025.2451242","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Comparison of pathogenicity factors of avian pathogenic and extraintestinal pathogenic Escherichia coli isolates originating from broiler chickens.
1. E. coli is an opportunist pathogen of animals, including food-producing ones and humans. Chickens may be a notable source of pathogenic and antimicrobial resistant E. coli for transmission to humans.2. This study compared virulence-associated genes (VGs) and antimicrobial resistance (AMR) in avian pathogenic E. coli (APEC) and extraintestinal pathogenic E. coli (ExPEC) isolates from broiler chickens, specifically APEC isolates in liver samples (n = 78) and ExPEC or non-ExPEC isolates in litter samples (n = 34). Virulence was evaluated by PCR for feoB, hlyF, iroN, iss, iutA and ompT genes, while AMR was evaluated by using antimicrobials from seven classes and detecting blaSHV, blaTEM, blaOXA, qnrB, stcM, mrc1, mrc2, sul1 and tetA genes.3. The APEC isolates were found in 100% of livers, while ExPEC and non-ExPEC isolates were found in 44% and 56% of the litter samples. The predominant VG was feoB (100%), followed by ompT (63%), iutA (60%), iss (58%) and hlyF (43%). Surprisingly, iroN, omp T and iutA had higher prevalences in APEC isolates (85%, 96% and 96%, respectively) than in ExPEC isolates (73%, 87% and 73%, respectively) and non-ExPEC isolates (0% for all). The presence of all VG in 33% of isolates indicated high pathogenicity.4. The isolates were phenotypically resistant to ampicillin (93%), ceftazidime (72%) and nalidixic acid (82%). All APEC and ExPEC isolates (100%) were multidrug resistant (MDR), while 63% of non-ExPEC isolates were MDR. Genotypic AMR testing revealed that 53% and 52% of all isolates had stcM and tetA, respectively. No isolate was positive for blaSHV, blaOXA, mrc1 or mrc2, which suggested the benefits of colistin for treating carbapenem-resistant enteric pathogens, due to the high resistance detected to meropenem (47%).5. Given the potential pathogenicity of E. coli isolates, improving biosecurity practices in chicken flocks should be prioritised to eliminate transmission to humans through the food chain.
期刊介绍：
From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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