急性砷暴露可诱导大鼠细胞遗传毒性和组织学改变。

IF 3.6 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Trace Elements in Medicine and Biology Pub Date : 2025-01-16 DOI:10.1016/j.jtemb.2025.127600

Fakhira Khalid, Hamda Azmat

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引用次数: 0

摘要

背景：砷是美国有毒物质和疾病登记处（ATSDR）名单上排名第一的最具毒性的有害元素，它很容易在鱼类体内积累，通过食用输送给人类，并影响人类和水生生物。综上所述，本实验旨在评价砷对罗氏Labeo rohita的细胞遗传毒性和组织学改变。方法：采用完全随机设计取样法，将驯化的罗氏Labeo个体（10批，每批10只，3个重复）在玻璃水族箱中暴露于9个确定剂量（12、14、16、18、20、22、24、26和28 mgL-1）的砷，测定其96 h致死浓度（LC50）。不含砷的对照组也同时运行。各组暴露96 h后进行组织生化指标测定。结果：砷96 h致死浓度为20.2 mgL-1。砷暴露后，氧化应激生物标志物：过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和脂质过氧化（LPO）以及所有靶器官中砷的积累均以剂量依赖方式显著增加（p ≤ 0.05），与未暴露组（对照组）相比。砷暴露后血清肝功能酶、免疫状态（白蛋白、球蛋白和总蛋白）、皮质醇水平和细胞色素P450基因表达显著改变（p ≤ 0.05）。组织学分析还显示，暴露于砷后，鳃和肝组织发生了破坏性改变。结论：这些结果证实，砷暴露导致Labeo rohita明显的有害改变，并证明有必要监测砷浓度的惊人增加。

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Acute arsenic exposure induces cyto-genotoxicity and histological alterations in Labeo rohita

Background

Arsenic emerges as most potent hazardous element ranked as number one in ATSDR (Agency for Toxic Substances and Disease Registry) list, can easily accumulate in fish, transported to humans via consumption and affect humans and aquatic organisms. Considering above, current experiment designed to evaluate cyto-genotoxicity and histological alterations induced by arsenic in Labeo rohita used as an animal model.

Methods

By applying complete randomized design sampling acclimatized individuals of Labeo rohita (10 batches of 10 each with triplicates) were exposed to nine definitive doses (12, 14, 16, 18, 20, 22, 24, 26 and 28 mgL⁻¹) of arsenic in glass aquaria to determine 96-h lethal concentration (LC₅₀) of arsenic. Control group without arsenic was also run simultaneously. After 96-h exposure various histo-biochemical parameters were evaluated in all experimental groups.

Results

The 96-h lethal concentration of arsenic was found to be 20.2 mgL⁻¹. Upon arsenic exposure, oxidative stress biomakers: catalase (CAT), superoxide dismutase (SOD) and lipid per oxidation (LPO) and accumulation of arsenic in all targeted organs were considerably (p ≤ 0.05) increased in dose dependent manner and in comparison, to unexposed (control) group. Serum liver function enzymes, immunological status (albumin, globulin and total protein), cortisol level and cytochrome P450 gene expression remarkably (p ≤ 0.05) altered on arsenic exposure. The histological analysis also showed destructive alterations on exposure to arsenic in gill and liver tissues.

Conclusion

These results confirmed that exposure of arsenic led to pronounced deleterious alterations in Labeo rohita and evidencing the need for monitoring alarmingly increasing concentration of arsenic.

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来源期刊

Journal of Trace Elements in Medicine and Biology 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.90%

发文量

202

审稿时长

85 days

期刊介绍： The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.