登革热病毒血清型和基因型鉴定：比哈尔邦巴特那AIIMS的综合研究。

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI:10.1016/j.ijmmb.2025.100789

Kamal Singh , Md Zeeshan Farooque , Vikram Pal Gandhi, Apurva, Aman Kumar, Asim Sarfraz, Binod Kumar Pati

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引用次数: 0

摘要

目的：登革热病毒是一个主要的全球健康问题，具有显著的遗传多样性，导致不同的血清型和基因型。登革热是仅次于疟疾的第二大由蚊子传播并感染人类的疾病。近几十年来，病毒传播的趋势也从城市向城郊和农村环境发生了转变。本研究的重点是登革热病毒血清型和基因型的鉴定和表征。方法：对一年内采集的标本进行NS1和IgM血清学检测，然后对NS1阳性标本进行流行登革热病毒血清型和基因型鉴定和鉴定。结果：2021年共检测血清933份，其中75份血清NS1抗原阳性，135份血清IgM抗体阳性。登革热血清型特异性Real-Time RT-PCR检测在ns1阳性样本中检测到所有4种血清型，表明该地区存在登革热血清型。其中，4例（7.8%）检出DEN-1, 26例（50.9%）检出DEN-2, 20例（39%）检出DEN-3, 1例（1.9%）检出DEN-4。在51例real - time RT-PCR阳性样本中，33例通过靶向衣壳-膜前（C-prM）区域的常规PCR基因分型发现阳性。基因分型结果显示，DENV-1血清型与GIII聚集，denv -2血清型与IVa、IVb、IVc等多种基因型聚集，denv -3血清型与GIII基因型聚集，denv -4血清型为GIII基因型。结论：本研究提供了该地区流行血清型和流行基因型的最新细节。

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Identification of dengue virus serotype and genotype: A comprehensive study from AIIMS Patna, Bihar

Purpose

Dengue virus, a major global health concern, exhibits significant genetic diversity, leading to distinct serotypes and genotypes. Dengue is the second most common disease spread by mosquitoes that infect humans, after malaria. In recent decades, there has also been a shift in the tendencies of virus transmission from urban to peri-urban and rural settings. This research study focuses on the identification and characterization of dengue virus serotypes and genotypes.

Methods

In this study, specimens collected in one year were first subjected to serological assay (NS1 and IgM) and subsequently the NS1 positive samples were subjected to identification and characterization of the prevalent dengue virus serotypes and genotypes.

Results

During the year 2021, a total of 933serum samples were tested, out of which 75 were found positive for NS1 antigen and 135 were positive for Dengue IgM antibody by ELISA. The dengue serotype specific Real-Time RT-PCR Assay detected all four serotypes in the NS1-positive samples, indicating their presence in this region. Of these, DEN-1 was detected in 4 (7.8 %), DEN-2 in 26 (50.9 %), DEN-3 in 20 (39 %), DEN-4 in 1 (1.9 %). Out of 51Real‐Time RT‐PCR positive samples, 33 were found positive by conventional PCR for genotyping by targeting the capsid-preMembrane (C-prM)region. The genotyping result showed that DENV-1 serotype clustering with GIII, DEN-2 serotype clustering withwide range of genotypes such as IVa, IVb, and IVc, DEN-3 clustering with GIII genotype and DEN-4 serotype showed GIII genotype.

Conclusions

This study provides the recent details about the circulating serotypes along with prevalent genotypes in this region.

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来源期刊

Indian Journal of Medical Microbiology IMMUNOLOGY-

CiteScore

2.20

自引率

0.00%

发文量

154

审稿时长

73 days

期刊介绍： Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study. Review articles, Special Articles or Guest Editorials are accepted on invitation.