Kiran Fatima, Syed Zeeshan Haider Naqvi, Hazrat Ali, Noor Hassan, Farheen Ansari, Sidrah Saleem, Shah Jahan, Mushtaq Ahmad, Aniqa Nawaz, Anam Saqib
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In addition, the whole-genome evaluation of a Acinetobacter baumannii UOL-KIMZ-24-2 was carried out using various Bioinformatics tools. A total of 150 strains of A. baumannii were recovered and identified in the current study. Among them, 49% strains were found resistant to carbapenem. The bla<sub>OXA-51</sub> was found prevalent in the genome of A. baumannii recovered from medical ICU (38%). In addition, the UOL-KIMZ-24-2 genome analysis based on multilocus sequence typing (MLST) highlighted that UOL-KIMZ-24-2 belonged to ST2 (Pasteur scheme) sequence type. A total of 29 antimicrobial resistance (AMR) genes were present, importantly, bla<sub>OXA-66</sub>, bla<sub>OXA-23</sub> and bla<sub>OXA-25</sub>. The mobile genetic elements (MGEs) were identified as transposases and belonged to four classes e.g. IS15d1, ISAba24, ISEc29, and ISEc35. A total of 14 virulence factors encoded by 58 different genes were detected in UOL-KIMZ-24-2. 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引用次数: 0
摘要
耐碳青霉烯鲍曼不动杆菌（CRAB）是许多国家（主要是南亚国家）卫生保健机构面临的新威胁。本研究旨在对拉合尔拉合尔总医院（Lahore General Hospital）患者的螃蟹（CRAB）菌株进行全基因组鉴定和鉴定，并对其基因组前噬菌体进行鉴定。收集了200多个样品，并通过形态学和生化试验进行了鉴定。采用Kirby-Bauer法对这些菌株进行综合药敏评价，并通过对blaOXA-51的探索进一步确认为CRAB菌株。此外，利用各种生物信息学工具对鲍曼不动杆菌UOL-KIMZ-24-2进行了全基因组评估。本研究共检出150株鲍曼不动杆菌。其中49%的菌株对碳青霉烯类耐药。医学ICU康复鲍曼不动杆菌基因组中普遍存在blaOXA-51（38%）。此外，基于多位点序列分型（MLST）的UOL-KIMZ-24-2基因组分析表明，UOL-KIMZ-24-2属于ST2 （Pasteur scheme）序列型。共检测到29个耐药基因，主要为blaOXA-66、blaOXA-23和blaOXA-25。这些移动遗传元件（MGEs）被鉴定为转座酶，分别属于IS15d1、ISAba24、ISEc29和ISEc35四个类别。在UOL-KIMZ-24-2中共检测到58个不同基因编码的14个毒力因子。此外，在UOL-KIMZ-24-2基因组中鉴定出噬菌体序列，并将其划分为3个区域。结果表明，UOL-KIMZ-24-2含有AMR基因、MGEs基因和MGEs基因。噬菌体序列与毒力基因。
Whole-genome evaluation and prophages characterization associated with genome of carbapenem-resistant Acinetobacter baumannii UOL-KIMZ-24-2.
Carbapenem-resistant Acinetobacter baumannii (CRAB) is an emerging threat to healthcare settings in many countries, principally in South Asia. The current study was aimed to identify, evaluate whole-genome and characterize the prophages in genome of CRAB strain, recovered from patients of Lahore General Hospital, Lahore. More than 200 samples were collected and identified by morphological and biochemical tests. These strains were also subjected to a comprehensive antimicrobial susceptibility evaluation using Kirby-Bauer method and further confirmed as CRAB strains by exploring blaOXA-51. In addition, the whole-genome evaluation of a Acinetobacter baumannii UOL-KIMZ-24-2 was carried out using various Bioinformatics tools. A total of 150 strains of A. baumannii were recovered and identified in the current study. Among them, 49% strains were found resistant to carbapenem. The blaOXA-51 was found prevalent in the genome of A. baumannii recovered from medical ICU (38%). In addition, the UOL-KIMZ-24-2 genome analysis based on multilocus sequence typing (MLST) highlighted that UOL-KIMZ-24-2 belonged to ST2 (Pasteur scheme) sequence type. A total of 29 antimicrobial resistance (AMR) genes were present, importantly, blaOXA-66, blaOXA-23 and blaOXA-25. The mobile genetic elements (MGEs) were identified as transposases and belonged to four classes e.g. IS15d1, ISAba24, ISEc29, and ISEc35. A total of 14 virulence factors encoded by 58 different genes were detected in UOL-KIMZ-24-2. In addition, the phage sequences were identified in genome of UOL-KIMZ-24-2, divided into 3 regions. In conclusion, UOL-KIMZ-24-2 contained a mixture of AMR genes, MGEs. prophages sequences and virulence genes.
期刊介绍：
Current Genetics publishes genetic, genomic, molecular and systems-level analysis of eukaryotic and prokaryotic microorganisms and cell organelles. All articles are peer-reviewed. The journal welcomes submissions employing any type of research approach, be it analytical (aiming at a better understanding), applied (aiming at practical applications), synthetic or theoretical.
Current Genetics no longer accepts manuscripts describing the genome sequence of mitochondria/chloroplast of a small number of species. Manuscripts covering sequence comparisons and analyses that include a large number of species will still be considered.
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