Toxicity mechanism of metal-organic framework HKUST-1 and its carbonized product to Tetradesmus obliquus: Physiological and transcriptomic analysis

Metal-organic frameworks (MOFs) are emerging materials with unique structures and properties, which have been widely used in many fields due to their various advantages. However, compared with its popular application research, the ecological safety of MOFs has rarely been reported. In this paper, a biological model, the common freshwater green algae Tetradesmus obliquus (T. obliquus) was used to study the effects of the copper-based MOF HKUST-1 and its carbonation product DHKUST-1 on the physiology and transcription level of the algae. A suite of advanced material characterization techniques has been utilized to multidimensionally reveal the physicochemical properties of HKUST-1 and its carbonation product. Notably, DHKUST-1 exhibit higher stability than HKUST-1 in aqueous environments, with lower ion release. During a 96-h exposure experiment, relevant indicators such as algae density, chlorophyll-a content and antioxidant enzyme activities were measured. Additionally, an intriguing IBR model was employed to comprehensively assess the toxicity of HKUST-1 and DHKUST-1 on the antioxidant system of T. obliquus. Furthermore, an in-depth analysis was conducted on the differential gene expression changes in T. obliquus under 10 mg/L HKUST-1 stress, exploring the impact on various pathways within algal cells. Briefly, the toxicity mechanism of HKUST-1 on T. obliquus is multi-involved. The findings of this study are expected to provide important basic data and references for the evaluation of the ecological safety of MOFs.