补充叶酸对大鼠缺血再灌注肾损伤的影响:叶酸的预防作用

IF 2.6 4区 医学 Q2 PHYSIOLOGY
Journal of Physiological Sciences Pub Date : 2024-01-01 Epub Date: 2025-01-02 DOI:10.1186/s12576-024-00900-z
Aya E H Hamed, Sherif Khedr, Elsayed Ghonamy, Faten A Mahmoud, Mona A Ahmed
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引用次数: 0

摘要

叶酸(FA)具有抗炎和抗氧化的特性,可能对缺血再灌注(IR)损伤有保护作用。本研究探讨了FA是否通过靶向关键炎症介质高迁移率组盒-1 (HMGB1)来保护大鼠肾脏免受IR。50只成年雄性Wistar大鼠随机分为对照组、IR组、IR + FA预处理组和单独FA组。与对照组相比,IR显著损害肾功能,升高丙二醛、HMGB1、NF-κB和caspase 3的水平。FA预处理有效地逆转了这些有害的变化,保护肾功能并将组织损伤降至最低。单独服用FA组与对照组比较无显著差异,说明FA治疗无不良反应。在机制上,FA抑制HMGB1的表达及其下游NF-κB和caspase 3的激活,从而抑制炎症和细胞死亡。FA通过抑制hmgb1介导的炎症和细胞凋亡来保护大鼠肾脏免受ir诱导的损伤,这提示了一种潜在的治疗ir相关肾损伤的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of folic acid supplementation on ischemia‒reperfusion-induced kidney injury in rats: folic acid prophylactic role revisited.

Folic acid (FA), with its anti-inflammatory and antioxidant properties, may offer protection against ischemia-reperfusion (IR) injury. This study investigated whether FA safeguards rat kidneys from IR by targeting high mobility group box-1 (HMGB1), a key inflammatory mediator. Fifty adult male Wistar rats were randomly allocated into four groups: control, IR, IR + FA pretreatment, and FA alone. Compared to controls, IR significantly impaired renal function and elevated levels of malondialdehyde, HMGB1, NF-κB, and caspase 3. FA pretreatment effectively reversed these detrimental changes, protecting renal function and minimizing tissue damage. The FA-alone group showed no significant differences compared to the control group, indicating no adverse effects of FA treatment. Mechanistically, FA inhibited HMGB1 expression and its downstream activation of NF-κB and caspase 3, thereby quelling inflammation and cell death. FA shields rat kidneys from IR-induced injury by suppressing HMGB1-mediated inflammation and apoptosis, suggesting a potential therapeutic avenue for IR-associated kidney damage.

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来源期刊
CiteScore
4.40
自引率
4.30%
发文量
27
审稿时长
6-12 weeks
期刊介绍: The Journal of Physiological Sciences publishes peer-reviewed original papers, reviews, short communications, technical notes, and letters to the editor, based on the principles and theories of modern physiology and addressed to the international scientific community. All fields of physiology are covered, encompassing molecular, cellular and systems physiology. The emphasis is on human and vertebrate physiology, but comparative papers are also considered. The process of obtaining results must be ethically sound. Fields covered: Adaptation and environment Autonomic nervous function Biophysics Cell sensors and signaling Central nervous system and brain sciences Endocrinology and metabolism Excitable membranes and neural cell physiology Exercise physiology Gastrointestinal and kidney physiology Heart and circulatory physiology Molecular and cellular physiology Muscle physiology Physiome/systems biology Respiration physiology Senses.
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