尿氧化应激标志物8-羟基鸟苷在尿样中的日波动。

IF 2.7 4区 医学 Q2 GENETICS & HEREDITY
Yun-Shan Li, Koichi Fujisawa, Kazuaki Kawai
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引用次数: 0

摘要

背景:尿8-羟基鸟苷(8-OHGuo)水平可作为氧化应激和羟基自由基诱导的RNA损伤的生物标志物。评估尿8- oh - guo排泄水平的日波动对于理解其含义至关重要。然而,这方面的研究仍然有限。在这项研究中,我们的目的是研究8-OHGuo水平的日波动以及影响这些变化的因素,使用尿样。方法:收集7名健康受试者从醒来时间至24时每次排尿的尿液样本,评估其昼夜变化。为了评估每日波动,从18名健康参与者连续23天醒来时收集尿液样本。采用HPLC-ECD法测定尿8-OHGuo水平。结果:未观察到非吸烟者尿8-OHGuo日水平的显著变化。相反,吸烟者尿液中8-OHGuo的日变化显著,变异系数为18.71%。尽管有一些日波动,但每个个体都保持一个特征值。此外,8-OHGuo的日水平受到生活方式因素的影响,包括精神状态、睡眠时间、吸烟、月经周期和饮食习惯。结论:8-OHGuo作为RNA氧化的特异性标记,与广泛使用的DNA氧化标记8-羟基脱氧鸟苷作为氧化应激的指标不同,提供了独特的见解。尿液8-OHGuo可以作为一种有价值的生物标志物,用于管理和预防氧化应激相关疾病,前提是确定了每日变化的特定范围。尿8-OHGuo水平的高日变化需要使用多个样本来准确测定个体水平。然而,进一步的大样本研究将有助于验证这些发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samples.

Background: Urinary 8-hydroxyguanosine (8-OHGuo) levels serve as a biomarker for oxidative stress and hydroxyl radical-induced RNA damage. Evaluating the diurnal and daily fluctuations in urinary 8-OHGuo excretion levels is essential for understanding its implications. However, research in this area remains limited. In this study, we aim to investigate the diurnal and daily fluctuations in 8-OHGuo levels as well as the factors that influence these variations, using spot urine samples.

Methods: Urine samples were collected from seven healthy participants during each urination from the time of awakening until 24:00 h to evaluate diurnal variations. To assess daily fluctuations, urine samples were collected from 18 healthy participants at the time of awakening for 23 consecutive days. The urinary 8-OHGuo levels were measured using an HPLC-ECD method.

Results: No significant variations were observed in the diurnal levels of urinary 8-OHGuo among non-smokers. Conversely, the daily variation of 8-OHGuo in the urine of the smoker was significant, with a coefficient of variation of 18.71%. Each individual maintained a characteristic value despite some diurnal fluctuations. Furthermore, the daily levels of 8-OHGuo exhibited a range of variations influenced by lifestyle factors, including mental state, sleep duration, smoking, menstrual cycle, and dietary habits.

Conclusion: As a specific marker of RNA oxidation, 8-OHGuo provides unique insights distinct from those provided by the widely used DNA oxidation marker 8-hydroxydeoxyguanosine as an indicator of oxidative stress. Urinary 8-OHGuo could serve as a valuable biomarker for managing and preventing oxidative stress-related diseases, provided that the specific range of daily variations is established. The high daily variation in urinary 8-OHGuo levels necessitates the use of multiple samples to accurately determine individual levels. However, further research with large sample sizes will help to validate these findings.

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来源期刊
Genes and Environment
Genes and Environment Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
4.00
自引率
0.00%
发文量
24
审稿时长
27 weeks
期刊介绍: Genes and Environment is an open access, peer-reviewed journal that aims to accelerate communications among global scientists working in the field of genes and environment. The journal publishes articles across a broad range of topics including environmental mutagenesis and carcinogenesis, environmental genomics and epigenetics, molecular epidemiology, genetic toxicology and regulatory sciences. Topics published in the journal include, but are not limited to, mutagenesis and anti-mutagenesis in bacteria; genotoxicity in mammalian somatic cells; genotoxicity in germ cells; replication and repair; DNA damage; metabolic activation and inactivation; water and air pollution; ROS, NO and photoactivation; pharmaceuticals and anticancer agents; radiation; endocrine disrupters; indirect mutagenesis; threshold; new techniques for environmental mutagenesis studies; DNA methylation (enzymatic); structure activity relationship; chemoprevention of cancer; regulatory science. Genetic toxicology including risk evaluation for human health, validation studies on testing methods and subjects of guidelines for regulation of chemicals are also within its scope.
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