硅酸盐取代的牛源羟基磷灰石在再生牙科中的骨替代物。

IF 3.1 4区 医学 Q2 BIOPHYSICS
Jithendra Ratnayake, Niranjan Ramesh, Maree L Gould, Michael R Mucalo, George J Dias
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引用次数: 0

摘要

羟基磷灰石以其生物相容性和骨导电性而闻名,由于其与天然骨矿物的相似性,在骨再生中起着重要作用,因此在推进组织工程策略方面具有相当大的潜力。本文报道了通过定制的溶胶-凝胶工艺将硅离子整合到生物源(牛源)羟基磷灰石(SiBHA)中的创新方法。所制得的SiBHA支架具有相互连接的微孔结构,总孔隙率为70%,孔径为120 ~ 650µm。傅里叶变换红外光谱和x射线衍射研究证实了硅离子在BHA晶格中的有效结合,能量色散x射线和电感耦合等离子体质谱进一步证实了SiBHA的Ca/P摩尔比在1.63至1.74之间。此外,SiBHA支架具有良好的化学和热稳定性。值得注意的是,与对照BHA支架相比,SiBHA支架显示出显著增强的力学性能,包括抗压强度和杨氏模量。体外评估强调了SiBHA支架促进Saos-2细胞活力、增殖和成骨分化的能力。免疫组织化学分析显示,在成骨条件下培养14天后,骨连接蛋白(一种关键的骨基质蛋白)的表达显著增加。这些发现突出了SiBHA支架的生物相容性和治疗潜力,表明其作为牙骨再生生物材料的适用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Silicate-substituted bovine-derived hydroxyapatite as a bone substitute in regenerative dentistry.

Hydroxyapatite, renowned for its biocompatibility and osteoconductive properties, plays a fundamental role in bone regeneration owing to its resemblance to natural bone mineral, thus offering considerable potential for advancing tissue engineering strategies. In this article, the innovative integration of silicon ions into biogenic (bovine-derived) hydroxyapatite (SiBHA) via a tailored sol-gel process is reported. The resultant SiBHA scaffolds exhibited an interconnected microporous structure with a total porosity of 70% and pore dimensions ranging from 120 to 650 µm. Fourier-transform infrared spectroscopy and X-ray diffraction studies validated the effective incorporation of silicon ions into the BHA lattice, with energy-dispersive X-ray and inductively-coupled plasma mass spectrometry further confirming a Ca/P molar ratio for SiBHA between 1.63 and 1.74. Moreover, SiBHA scaffolds demonstrated commendable chemical and thermal stability. Of note, SiBHA scaffolds were found to display significantly enhanced mechanical properties, including compressive strength and Young's modulus, compared to the control BHA scaffolds. In vitro assessments highlighted the capacity of SiBHA scaffolds to foster cell viability, proliferation, and osteogenic differentiation of Saos-2 cells. Immunohistochemical analysis revealed a significant increase in osteonectin expression, a key bone matrix protein, after 14 days of incubation under osteogenic conditions. These findings highlight the biocompatibility and therapeutic potential of SiBHA scaffolds, suggesting their suitability as biomaterials for dental bone regeneration applications.

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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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