薤白葡甘露聚糖的结构特征及初步免疫活性研究。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-01-03 DOI:10.1016/j.carres.2025.109375

Huiwen Li , Aigen Fu , Heping Hui , Feng Jia , Huan Wang , Tong Zhao , Jing Wei , Peijuan Zhang , Wuying Lang , Kejuan Li , Xuansheng Hu

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引用次数: 0

摘要

采用离子渗透层析和凝胶渗透层析纯化，从Allii Tuberosi Semen中分离得到一种新的葡甘露聚糖ATSW-1。采用高效色谱、傅里叶变换红外光谱、气相色谱-质谱联用、核磁共振谱等方法对其结构进行了表征。采用细胞计数试剂盒-8、中性红吞噬实验及相关细胞因子的分泌检测ATSW-1的体外免疫活性。结果表明，ATSW-1的分子量为35.9 kDa，主要由甘露糖、葡萄糖和阿拉伯糖组成，摩尔比为1:0.72:0.04。它的主要支柱是由→4)-β-D-Glcp -(1→→4)-β-D-Manp -(1→→4,6)-α-D-Manp -β(1→,分支-D-Glcp -(1→α-L-Araf -(1→→5)α-L-Araf -(1→链接替换在其他位置→4)-α-D-Manp -(1→。ATSW-1显著增强免疫应答，RAW264.7细胞的吞噬活性较空白对照组提高1.52倍。进一步的研究表明，ATSW-1通过上调NO、TNF-α和IL-6的分泌来促进免疫活性。这些研究结果为其在功能性食品或免疫相关药物中的潜在开发和应用奠定了基础。

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Structure characterization and preliminary immune activity of a glucomannan purified from Allii Tuberosi Semen

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Structure characterization and preliminary immune activity of a glucomannan purified from Allii Tuberosi Semen

A novel glucomannan, named ATSW-1, was obtained from Allii Tuberosi Semen by ion and gel permeation chromatography purification. Its structure was characterized using high-performance chromatography, Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy. The in vitro immune activities of ATSW-1 were examined using the Cell Counting Kit-8, the neutral red phagocytosis assay, and the secretion of related cytokines. The results showed that the molecular weight of ATSW-1 was 35.9 kDa and mainly constituted of mannose, glucose, and arabinose with a molar ratio of 1:0.72:0.04. Its main backbone was composed of →4)-β-D-Glcp-(1→, →4)-β-D-Manp-(1→, and →4,6)-α-D-Manp-(1→, with the branches β-D-Glcp-(1→, α-L-Araf-(1→ and →5)-α-L-Araf-(1→ linkage substituted at C-6 position of →4)-α-D-Manp-(1 → . ATSW-1 significantly enhanced immune responses, increasing the phagocytic activity of RAW264.7 cells by 1.52 times compared to the blank control group. Further investigations revealed that ATSW-1 promotes immune activity by upregulating the NO, TNF-α, and IL-6 secretion. These findings provide a foundation for the potential development and application of Allii Tuberosi Semen polysaccharides in functional foods or immune-related therapeutics.

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".