Wenhui Yuan, Yuanzheng Qiu, Qinglai Tang, Mengmeng Li, Xiaojun Tang, Tao Yang
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引用次数: 0
摘要
目的：本研究旨在探讨m6a相关长链非编码rna （lncRNAs）在头颈部鳞状细胞癌（HNSCC）预后和肿瘤微环境中的作用。方法：分析来自The Cancer Genome Atlas的497个样本，通过相关模型鉴定m6a相关的lncrna。然后利用三方回归模型、Kaplan-Meier分析和norm图来评估这些lncrna的预后意义。还进行了肿瘤突变负荷和免疫细胞浸润分析。RT-qPCR证实m6a相关lncRNAs的表达及与IGF2BP2的关系。结果：风险模型显示，高风险评分预测较差的生存结果。在训练集中，预测1、3、5年生存的ROC曲线下面积分别为0.70、0.68和0.64。7个关键的m6a相关lncrna显示与免疫检查点分子相关，特别是CTLA4和PD-1。最后，我们发现敲低TUG1可抑制IGF2BP2的表达。结论：我们的研究结果表明m6a相关的lncRNA风险模型在预测HNSCC患者的预后和免疫治疗反应方面具有潜在的临床应用价值。免疫治疗候选化合物的鉴定进一步强调了该模型在指导HNSCC治疗决策方面的相关性。
Identification of IGF2BP2 and long non-coding RNA TUG1 for the prognosis and tumour microenvironment in head and neck squamous cell carcinoma.
Objective: This study aimed to investigate the role of m6A-related long non-coding RNAs (lncRNAs) in the prognosis and tumour microenvironment of head and neck squamous cell carcinoma (HNSCC).
Methods: 497 samples from The Cancer Genome Atlas were analysed to identify m6A-related lncRNAs via correlation models. Tripartite regression models, Kaplan-Meier analysis and nomograms were then utilised to assess the prognostic significance of these lncRNAs. Tumour mutation burden and immune cell infiltration analyses were also performed. Moreover, m6A-related lncRNAs expression and relation with IGF2BP2 were confirmed by RT-qPCR.
Results: The risk model revealed that high-risk scores predicted poorer survival outcomes. The area under ROC curves for predicting 1-, 3-, 5-year survival in the training set were 0.70, 0.68, and 0.64, respectively. Seven key m6A-related lncRNAs showed associations with immune checkpoint molecules, especially CTLA4 and PD-1. Finally, we found that knockdown of TUG1 repressed the expression of IGF2BP2.
Conclusions: Our results suggest that the m6A-related lncRNA risk model has potential clinical utility in predicting prognosis and immunotherapeutic responses in patients with HNSCC. Identification of candidate compounds for immunotherapy further emphasises the model's relevance in guiding treatment decisions for HNSCC.
期刊介绍：
Acta Otorhinolaryngologica Italica first appeared as “Annali di Laringologia Otologia e Faringologia” and was founded in 1901 by Giulio Masini.
It is the official publication of the Italian Hospital Otology Association (A.O.O.I.) and, since 1976, also of the Società Italiana di Otorinolaringoiatria e Chirurgia Cervico-Facciale (S.I.O.Ch.C.-F.).
The journal publishes original articles (clinical trials, cohort studies, case-control studies, cross-sectional surveys, and diagnostic test assessments) of interest in the field of otorhinolaryngology as well as clinical techniques and technology (a short report of unique or original methods for surgical techniques, medical management or new devices or technology), editorials (including editorial guests – special contribution) and letters to the Editor-in-Chief.
Articles concerning science investigations and well prepared systematic reviews (including meta-analyses) on themes related to basic science, clinical otorhinolaryngology and head and neck surgery have high priority.
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