Slow sand filters with variable filtration rates for rainwater purification: Microecological differences between biofilm and water phases

Slow sand filters (SSFs) have been increasingly applied to rainwater purification in recent years, but the response of SSFs to fluctuating rainfall, as well as the biofilm- and water-phase microecology in SSFs are still poorly understood. This study systematically evaluated the rainwater purification performance of SSFs and compared the bacterial community structure, assembly processes and molecular ecological interactions between the biofilm and water phases. The activated carbon and activated alumina filters exhibited the best performance for NH₄⁺-N (18.82%∼64.00%) and TP (>90%) removal, respectively. As the filtration rate increased from 0.1 m/h to 0.3 m/h, the rainwater purification efficiencies of the three SSFs deteriorated significantly, with the enrichment of Tolumonas, Desulfovibrio and Sulfurospirillum, and reduction in Klebsiella and Enterobacter. The community diversity of biofilm phase was significantly higher than that of water phase, and filtration rate was identified as a key factor in shaping the bacterial community in both phases. The interactions of filtration rate and water quality displayed the best and significant (p < 0.01) explanation for microbiome shift, with the higher values in biofilm phase (34.70%) than in water phase (24.02%). Bacterial community assembly in SSFs was determined by stochastic ecological processes, which played a more important role in water-phase communities, with 86.34% following predictions using a neutral community model. The molecular ecological network of biofilm phase exhibited more complexity, lower modularity and more cooperative relationships than that of water phase. Disadvantaged OTUs occupied core and notable positions in the network, with the highest degree and clustering coefficient. Different keystone species were identified in biofilm- (Runella, Aquabacterium, etc) and water-network (Terrimonas) respectively, despite they processed low relative abundances (<0.1%). These results enhance the understanding of microecology in SSFs, and shed new lights on the improvement and promotion of rainwater biological treatment technology.